AcH4 Antibody with Positive Primer Set

Details for Product No. ABIN3071717
Antigen
  • hist1h4l
  • H1ft
  • Hist4
  • Hist4h4
  • H4
  • X04652
  • FO108
  • H4/n
  • H4F2
  • H4FN
  • HIST2H4
  • H4C13
  • H4C7
  • F8N16.2
  • F8N16_2
  • histone H4
  • BcDNA:RH52884
  • CG3379
  • Dmel\CG3379
  • H4r
  • His4-88CD
  • His4R
  • histone cluster 1, H4a L homeolog
  • histone cluster 1 H4 family member B
  • histone cluster 2, H4
  • histone cluster 2 H4 family member a
  • histone H4
  • Histone H4 replacement
  • Histone H4
  • hist1h4a.L
  • Hist1h4b
  • Hist2h4
  • HIST2H4A
  • H4
  • H4C14
  • HIS4
  • HIST2H4
  • His4r
  • his-38
Epitope
acetylated
Reactivity
Human
10
5
4
4
1
Host
Sheep
Clonality
Monoclonal
Application
Chromatin Immunoprecipitation (ChIP)
Options
Purpose ChIP Validated AcH4 Antibody and Positive Primer Set provides a complete set of tools to assist with a successful ChIP assay.
Characteristics A sheep IgG is included in this Antibody Primer Set as a negative control for the ChIP experiment. AcH4 is associated with active transcription, the positive primer set included in this Antibody Primer Set recognises a gene that is present when transcription is switched on.
Components AcH4 antibody, control sheep IgG, and a positive primer set. Antibody Concentration: 1 mg/mL
Target Name (Antigen)
Background Histone 4 (H4) is one of the core histone proteins, comprising the protein component of chromatin. It is the smallest subunit of chromatin. Acetylation of histone H4 occurs at several different lysine positions on the histone tail and is catalysed by a group of enzymes called histone acetyltransferases (HATs). Histone acetylation is associated with active transcription.
Application Notes AcH4 2:1 (chromatin: antibody)
Sheep IgG 2:1 (chromatin: antibody)
Positive Primer Set Dilute from 4μM (provided) to 1μM working concentration
Note: Optimal dilutions should be determined by the user. These volumes are stated as guidelines only.
Comment

The ChIP Validated AcH4 Antibody and Positive Primer Set is not suitable for use with non-human species. Antibody is Protein G affinity purified.

Restrictions For Research Use only
Storage 4 °C,-20 °C
Storage Comment Avoid multiple freeze/thaw cycles as this may denature the antibody and degrade the primer sets
Supplier Images
Image no. 1 for AcH4 Antibody with Positive Primer Set (ABIN3071717) AcH4 ChIP qPCR: Chromatin immunoprecipitation (ChIP) assays were performed using the...