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Total Polyphenols Quantification Assay Kit

Q Colorimetric Beverages, Food Quantitative Uncoated
Catalog No. ABIN7384588
  • Detection Method
    Colorimetric
    Detection Range
    3.5-25 μg/mL
    Minimum Detection Limit
    3.5 μg/mL
    Application
    Quantification (Q)
    Purpose
    The kit is designed for the rapid determination of polyphenols in various samples.
    Sample Type
    Beverages, Food
    Analytical Method
    Quantitative
    Specificity
    Specific for phenol content determination.
    Characteristics
    Phenolic Quantification Assay is based on Folin-Ciocalteu method. The FC reagent contains phosphomolybdic/ phosphotungstic acid complexes1. The method relies on the transfer of electrons in alkaline medium from phenolic compounds to form a blue chromophor
    Components
    Plate and the reagents neccesary to perform the assay.
    Material not included
    Pipettes, reaction tubes, plate reader.
  • Comment

    1 ml (microassay)/ 20μL (microplate)

    Assay Time
    15 min
    Plate
    Uncoated
    Reagent Preparation

    Reagent A -FC reagent-needs to be diluted (1:10)

    Assay Procedure

    MICROASSAY: 1.Pipette 1 mL of each standard, unknown sample or diluted sample replicate into separate clean test tubes. Refer to the Table 1 (see booklet) as a guide for diluting the standard phenol. For the diluent, use the same buffer as in the samples. 2.Add 1 mL of Reagent A -Folin-Ciocalteu Reagent previously diluted (1:10) in deionized water- to each tube. 3.Add 1 mL of Reagent B (Alkaline Working Solution) to each tube and measure the absorbance at 700 nm immediately. 4.Measure the absorbance of these standards, blanks and unknown samples at 700 nm. MICROPLATE: 1. Prepare standards containing a range of 1 to 10 μg phenol (Gallic acid) to a volume of 200 μL. Pipette 20 μL of each standard and unknown sample or diluted sample replicate into a microplate well. Refer to the Table 2 (see booklet) as a guide for diluting the standard phenol. For the diluent, use the same buffer as in the samples. 2.To each well, add 100 μL of Reagent A -FC reagent-previously diluted (1:10) in deionized water. 3.Add 80 μL ofReagent B (Alkaline Working Solution) to each well and measure the absorbance at 700 nm on a plate reader immediately. 4.Measure the absorbance of these standards, blanks and unknown samples at 700 nm.

    Calculation of Results

    1.If the spectrophotometer or microplate reader was not zeroed with the blank, then substract the average blank value from the standard and unknown sample values. 2.Create a standard curve by plotting A700nm (y-axis) vs standard, μg (x-axis). Determine the unknown sample concentration using the standard curve. 3.Standard curve example for microplate assay procedure is shown in Figure 2 (see images). 4.Dilute the unknown samples until they reach an absorbance within the limits of the standard curve.

    Restrictions
    For Research Use only
  • Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT/4 °C
    Storage Comment
    FC Reagent A: RT, FC Reagent B: RT, FC Standard: 4 °C
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