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BMP2 ELISA Kit

BMP2 Reactivity: Mouse AA 283-396 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Osseous Tissue, Serum
Catalog No. ABIN411262
  • Target See all BMP2 ELISA Kits
    BMP2 (Bone Morphogenetic Protein 2 (BMP2))
    Binding Specificity
    AA 283-396
    Reactivity
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5-4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse BMP-2
    Brand
    PicoKine™
    Sample Type
    Osseous Tissue, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: CHO
    Immunogen sequence: Q283-R396
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Predicted Reactivity
    Bovine,Canine,Horse,Monkey,Rabbit
    Sensitivity
    <2pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: CHO
    Immunogen sequence: Q283-R396
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plate
    Pre-coated
    Protocol
    mouse BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-2 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse BMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse bone tissue, cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse BMP-2 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 314, Standard deviation: 11.3, CV(%): 3.6
    • Sample 2: n=16, Mean(pg/ml): 1002, Standard deviation: 39.1, CV(%): 3.9
    • Sample 3: n=16, Mean(pg/ml): 2263, Standard deviation: 88.3, CV(%): 3.9,
    • Sample 1: n=24, Mean(pg/ml): 334, Standard deviation: 24, CV(%): 7.2
    • Sample 2: n=24, Mean(pg/ml): 1059, Standard deviation: 74.1, CV(%): 7
    • Sample 3: n=24, Mean(pg/ml): 2360, Standard deviation: 177, CV(%): 7.5
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all BMP2 ELISA Kits
    BMP2 (Bone Morphogenetic Protein 2 (BMP2))
    Alternative Name
    BMP2 (BMP2 Products)
    Synonyms
    BDA2 ELISA Kit, BMP2A ELISA Kit, AI467020 ELISA Kit, Bmp2a ELISA Kit, BMP-2 ELISA Kit, xBMP-2 ELISA Kit, xbmp2 ELISA Kit, BMP2 ELISA Kit, bmp2a ELISA Kit, bmp2 ELISA Kit, wu:fc59d09 ELISA Kit, bone morphogenetic protein 2 ELISA Kit, bone morphogenetic protein 2 L homeolog ELISA Kit, Bone morphogenetic protein 2 ELISA Kit, bone morphogenetic protein 2a ELISA Kit, BMP2 ELISA Kit, Bmp2 ELISA Kit, bmp2.L ELISA Kit, bmp2 ELISA Kit, bmp2a ELISA Kit
    Background

    Protein Function: Induces cartilage and bone formation.

    Background: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant mouse BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

    Synonyms: Bone morphogenetic protein 2,BMP-2,Bone morphogenetic protein 2A,BMP-2A,Bmp2,Bmp-2,

    Full Gene Name: Bone morphogenetic protein 2

    Cellular Localisation: Secreted.
    Gene ID
    12156
    UniProt
    P21274
    Pathways
    Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Growth Factor Binding, Positive Regulation of fat Cell Differentiation
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