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CCL3 ELISA Kit

CCL3 Reactivity: Human AA 27-92 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Catalog No. ABIN411325
  • Target See all CCL3 ELISA Kits
    CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
    Binding Specificity
    AA 27-92
    Reactivity
    • 6
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 pg/mL
    Minimum Detection Limit
    15.6 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MIP-1 alpha
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: E.coli
    Immunogen sequence: A27-A92
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: A27-A92
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plate
    Pre-coated
    Protocol
    human MIP-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIP-1 alpha has been precoated onto 96-well plates. Standards(E.coli, A27-A92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MIP-1 alpha amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIP-1 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MIP-1 alpha standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 107, Standard deviation: 4.39, CV(%): 4.1
    • Sample 2: n=16, Mean(pg/ml): 450, Standard deviation: 13.05, CV(%): 2.9
    • Sample 3: n=16, Mean(pg/ml): 732, Standard deviation: 25.62, CV(%): 3.5,
    • Sample 1: n=24, Mean(pg/ml): 95, Standard deviation: 6.84, CV(%): 7.2
    • Sample 2: n=24, Mean(pg/ml): 413, Standard deviation: 22.3, CV(%): 5.4
    • Sample 3: n=24, Mean(pg/ml): 682, Standard deviation: 34.78, CV(%): 5.1
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CCL3 ELISA Kits
    CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
    Alternative Name
    CCL3 (CCL3 Products)
    Synonyms
    MIP-1alpha ELISA Kit, G0S19-1 ELISA Kit, LD78ALPHA ELISA Kit, MIP-1-alpha ELISA Kit, MIP1A ELISA Kit, SCYA3 ELISA Kit, MIP-1a ELISA Kit, Scya3 ELISA Kit, AI323804 ELISA Kit, LD78alpha ELISA Kit, MIP1-(a) ELISA Kit, MIP1-alpha ELISA Kit, Mip1a ELISA Kit, CCL3L1 ELISA Kit, CCL3L3 ELISA Kit, chemokine (C-C motif) ligand 3 ELISA Kit, C-C motif chemokine ligand 3 ELISA Kit, CCL3 ELISA Kit, Ccl3 ELISA Kit
    Background

    Background: Macrophage inflammatory protein-1 alpha(MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease. The standard product used in this kit is recombinant human MIP-1alpha, consisting of 66 amino acids with the molecular mass of 7.5 kDa.

    Synonyms: C-C motif chemokine ,CCL3 ,hCG_1749202 ,

    Full Gene Name: C-C motif chemokine

    Cellular Localisation: Secreted.
    Gene ID
    6348
    UniProt
    A0N0R1
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Autophagy
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