MMP 9 ELISA Kit

Catalog No. ABIN411333

Product Details MMP 9 ELISA Kit

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Binding Specificity: AA 20-707

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MMP-9

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Saliva, Urine

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: A20-D707

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <5pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A20-D707

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the peptidase M10A family.

Tissue Specificity: Produced by normal alveolar macrophages and granulocytes.

Plate: Pre-coated

Protocol: human MMP-9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-9 has been precoated onto 96-well plates. Standards(NSO, A20-D707) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MMP-9 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MMP-9 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.35, Standard deviation: 0.042, CV(%): 3.1
• Sample 2: n=16, Mean(ng/ml): 3.8, Standard deviation: 0.16, CV(%): 4.2
• Sample 3: n=16, Mean(ng/ml): 6.9, Standard deviation: 0.24, CV(%): 3.5,
• Sample 1: n=24, Mean(ng/ml): 1.7, Standard deviation: 0.095, CV(%): 5.6
• Sample 2: n=24, Mean(ng/ml): 3.9, Standard deviation: 0.308, CV(%): 7.9
• Sample 3: n=24, Mean(ng/ml): 7.1, Standard deviation: 0.511, CV(%): 7.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for MMP 9 ELISA Kit (ABIN411333)

Xiong, Liu, Zhu, Zhang, Qin, Yao, Zhou, Gao: "Precursor N-cadherin mediates glial cell line-derived neurotrophic factor-promoted human malignant glioma." in: Oncotarget, Vol. 8, Issue 15, pp. 24902-24914, (2018) (PubMed).

Sun, Yang, Xi, Chen, Fu, Sun, Yin, Li, Liu, Qin, Liu, Hou: "Primed atypical ductal hyperplasia-associated fibroblasts promote cell growth and polarity changes of transformed epithelium-like breast cancer MCF-7 cells via miR-200b/c-IKKβ signaling." in: Cell death & disease, Vol. 9, Issue 2, pp. 122, (2018) (PubMed).

Matin, Nemati, Ghobadi, Alipanah-Moghadam, Rezagholizadeh: "The effect of conjugated linoleic acid on oxidative stress and matrix metalloproteinases 2 and 9 in patients with COPD." in: International journal of chronic obstructive pulmonary disease, Vol. 13, pp. 1449-1454, (2018) (PubMed).

Liu, Bao, Zeng, Wei: "Effects of resveratrol and genistein on nuclear factor‑κB, tumor necrosis factor‑α and matrix metalloproteinase‑9 in patients with chronic obstructive pulmonary disease." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4266-72, (2017) (PubMed).

Jimenez Flores, Tian, Sizova, Epstein, Lamont, Uriarte: "Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis." in: Infection and immunity, Vol. 85, Issue 7, (2017) (PubMed).

Sun, Chen, Li, Lei, Xu, Jiang, Zhang, Cao, Ke: "NanoVelcro-captured CTC number concomitant with enhanced serum levels of MMP7 and MMP9 enables accurate prediction of metastasis and poor prognosis in patients with lung adenocarcinoma." in: International journal of nanomedicine, Vol. 12, pp. 6399-6412, (2017) (PubMed).

Hu, Ni, Cao, Zhang, Wu, Yin, Lang, Lu: "The Angiogenic Effect of microRNA-21 Targeting TIMP3 through the Regulation of MMP2 and MMP9." in: PLoS ONE, Vol. 11, Issue 2, pp. e0149537, (2016) (PubMed).

Qin, Li, Jiao, Fu, Ye, Ji, Xie, Fan, Xu: "Changes in Blood Components in Aphtha Patients with Excess Heat." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 7219257, (2016) (PubMed).

Bi, Zeng, Zhao, Wei, Yu, Wang, Yu, Cao, Shan, Wei: "miR-181a Induces Macrophage Polarized to M2 Phenotype and Promotes M2 Macrophage-mediated Tumor Cell Metastasis by Targeting KLF6 and C/EBPα." in: Molecular therapy. Nucleic acids, Vol. 5, Issue 9, pp. e368, (2016) (PubMed).

Chen, Yao, Zhu, Li, Guo, Yu: "Expression levels of matrix metalloproteinase-9 in human gastric carcinoma." in: Oncology letters, Vol. 9, Issue 2, pp. 915-919, (2015) (PubMed).

Stio, Martinesi, Treves, Borgioli: "In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments." in: Journal of materials science. Materials in medicine, Vol. 26, Issue 2, pp. 100, (2015) (PubMed).

Gishto, Farrell, Kothapalli: "Tuning composition and architecture of biomimetic scaffolds for enhanced matrix synthesis by murine cardiomyocytes." in: Journal of biomedical materials research. Part A, Vol. 103, Issue 2, pp. 693-708, (2015) (PubMed).

Liu, Zou, Zhou, Gong, Wang, Cai, Tan, Fang: "miR-155 Regulates Glioma Cells Invasion and Chemosensitivity by p38 Isforms In Vitro." in: Journal of cellular biochemistry, Vol. 116, Issue 7, pp. 1213-21, (2015) (PubMed).

Zhang, Jiang, Chen, Wang, Feng, Tao, Qin: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." in: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).

Lin, Qu, Geng, Li, Wu, Dong, Liu, Li: "Air exposure induced characteristics of dry eye in conjunctival tissue culture." in: PLoS ONE, Vol. 9, Issue 1, pp. e87368, (2014) (PubMed).

Mazani, Fard, Baghi, Nemati, Mogadam: "Effect of pomegranate juice supplementation on matrix metalloproteinases 2 and 9 following exhaustive exercise in young healthy males." in: JPMA. The Journal of the Pakistan Medical Association, Vol. 64, Issue 7, pp. 785-90, (2014) (PubMed).

Hopps, Lo Presti, Montana, Canino, Averna, Caimi: "Study of the correlations among some parameters of the oxidative status, gelatinases, and their inhibitors in a group of subjects with metabolic syndrome." in: Mediators of inflammation, Vol. 2014, pp. 510619, (2014) (PubMed).

Zawilla, Taha, Ibrahim: "Liver functions in silica-exposed workers in Egypt: possible role of matrix remodeling and immunological factors." in: International journal of occupational and environmental health, Vol. 20, Issue 2, pp. 146-56, (2014) (PubMed).

Yuan, Yang, Zhou, Gao, Qiu, Fang, Ding, Xiao et al.: "Knockdown of sphingosine kinase 1 inhibits the migration and invasion of human rheumatoid arthritis fibroblast-like synoviocytes by down-regulating the PI3K/AKT activation and MMP-2/9 production in ..." in: Molecular biology reports, Vol. 41, Issue 8, pp. 5157-65, (2014) (PubMed).

Li, Zhao, Ren, Xie, Ma, Rong: "Increased expression of matrix metalloproteinase-9 associated with gastric ulcer recurrence." in: World journal of gastroenterology, Vol. 19, Issue 28, pp. 4590-5, (2014) (PubMed).

Target Details for MMP 9

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Alternative Name: MMP9 (MMP9 Products)

Synonyms: CLG4B ELISA Kit, GELB ELISA Kit, MANDP2 ELISA Kit, MMP-9 ELISA Kit, AW743869 ELISA Kit, B/MMP9 ELISA Kit, Clg4b ELISA Kit, pro-MMP-9 ELISA Kit, mmp-9 ELISA Kit, fgMMP-9 ELISA Kit, mmp9 ELISA Kit, ZFMMP-9 ELISA Kit, wu:fb02g06 ELISA Kit, wu:fb07b05 ELISA Kit, wu:fi98c09 ELISA Kit, wu:fj05a08 ELISA Kit, zgc:64165 ELISA Kit, clg4b ELISA Kit, gelb ELISA Kit, mandp2 ELISA Kit, matrix metallopeptidase 9 ELISA Kit, collagenase ELISA Kit, matrix metalloproteinase 9 ELISA Kit, matrix metallopeptidase 9 S homeolog ELISA Kit, MMP9 ELISA Kit, RB3913 ELISA Kit, BPSS0666 ELISA Kit, Sbal_3732 ELISA Kit, Bcer98_0486 ELISA Kit, Shew185_0630 ELISA Kit, Shal_3056 ELISA Kit, Sbal195_0657 ELISA Kit, Lbys_3550 ELISA Kit, Palpr_2084 ELISA Kit, Mmp9 ELISA Kit, mmp9 ELISA Kit, mmp9.S ELISA Kit

Background:

Protein Function: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. .

Background: The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9(MMP9). The 72- and 92- kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases(MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema. The standard product used in this kit is recombinant human MMP-9 with the molecular mass of 95KDa. The detected MMP-9 includes zymogen and active enzyme.

Synonyms: Matrix metalloproteinase-9,MMP-9,3.4.24.35,92 kDa gelatinase,92 kDa type IV collagenase,Gelatinase B,GELB,67 kDa matrix metalloproteinase-9,82 kDa matrix metalloproteinase-9,MMP9,CLG4B,

Full Gene Name: Matrix metalloproteinase-9

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

Gene ID: 4318

UniProt: P14780

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events