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Phosphate Colorimetric Assay Kit Kit

BCA Reactivity: Chemical Colorimetric Cell Lysate, Plasma, Serum, Tissue Samples
Pubmed (14)
Catalog No. ABIN411732
$245.00
Plus shipping costs $45.00
500 tests
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target
    Phosphate
    Reactivity
    Chemical
    Detection Method
    Colorimetric
    Minimum Detection Limit
    0.1 nM
    Application
    Biochemical Assay (BCA)
    Sample Type
    Cell Lysate, Plasma, Serum, Tissue Samples
    Specificity
    The newly designed Phosphate Colorimetric Assay Kit provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around 650 nm. Phosphate concentrations between 1 μM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. The Phosphate Colorimetric Assay Kit provides 500 assays using microtiter plates or 100 assays using 1 mL cuvettes.
    Characteristics
    Phosphate Colorimetric Assay Kit: Easy, Quick and Sensitive Assay to Measure Phosphate level within 30 min.
    Components
    Phosphate Reagent
    Phosphate Standard (10 mM)
  • Application Notes
    Phosphate concentrations between 1 μM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
    Comment

    Further details regarding sample type: Cell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids, algal blooms and water from run-off areas of high fertilizer use

    Assay Time
    0.5 h
    Restrictions
    For Research Use only
  • Storage
    RT
    Expiry Date
    12 months
  • Prazeres, Uthicke, Pandolfi: "Ocean acidification induces biochemical and morphological changes in the calcification process of large benthic foraminifera." in: Proceedings. Biological sciences / The Royal Society, Vol. 282, Issue 1803, pp. 20142782, 2015 (PubMed).

    Wu-Wong, Chen, Wessale: "Vitamin D receptor agonist VS-105 improves cardiac function in the presence of enalapril in 5/6 nephrectomized rats." in: American journal of physiology. Renal physiology, Vol. 308, Issue 4, pp. F309-19, 2015 (PubMed).

    Burris, Webster, Sheriff, Faroqui, Levi, Hawse, Amlal: "Estrogen directly and specifically downregulates NaPi-IIa through the activation of both estrogen receptor isoforms (ERα and ERβ) in rat kidney proximal tubule." in: American journal of physiology. Renal physiology, Vol. 308, Issue 6, pp. F522-34, 2015 (PubMed).

    Krieger, Asplin, Frick, Granja, Culbertson, Ng, Grynpas, Bushinsky: "Effect of Potassium Citrate on Calcium Phosphate Stones in a Model of Hypercalciuria." in: Journal of the American Society of Nephrology : JASN, 2015 (PubMed).

    Chang, Chou, Hsu, Wang: "Proposed carrier lipid-binding site of undecaprenyl pyrophosphate phosphatase from Escherichia coli." in: The Journal of biological chemistry, Vol. 289, Issue 27, pp. 18719-35, 2014 (PubMed).

    Chen, Lin, Wu, Ho, Santhanam, Chou, Meng, Wang: "Reciprocal allosteric regulation of p38γ and PTPN3 involves a PDZ domain-modulated complex formation." in: Science signaling, Vol. 7, Issue 347, pp. ra98, 2014 (PubMed).

    Riddle, Frey, Tomlinson, Ferron, Li, DiGirolamo, Faugere, Hussain, Karsenty, Clemens: "Tsc2 is a molecular checkpoint controlling osteoblast development and glucose homeostasis." in: Molecular and cellular biology, Vol. 34, Issue 10, pp. 1850-62, 2014 (PubMed).

    Frick, Asplin, Culbertson, Granja, Krieger, Bushinsky: "Persistence of 1,25D-induced hypercalciuria in alendronate-treated genetic hypercalciuric stone-forming rats fed a low-calcium diet." in: American journal of physiology. Renal physiology, Vol. 306, Issue 9, pp. F1081-7, 2014 (PubMed).

    Maldonado, Sheldon, DeHart, Patnaik, Manevich, Townsend, Bezrukov, Rostovtseva, Lemasters: "Voltage-dependent anion channels modulate mitochondrial metabolism in cancer cells: regulation by free tubulin and erastin." in: The Journal of biological chemistry, Vol. 288, Issue 17, pp. 11920-9, 2013 (PubMed).

    Król, Biswas, King, Biswas: "SMU.746-SMU.747, a putative membrane permease complex, is involved in aciduricity, acidogenesis, and biofilm formation in Streptococcus mutans." in: Journal of bacteriology, Vol. 196, Issue 1, pp. 129-39, 2013 (PubMed).

    Keller, Schut, Lipscomb, Menon, Iwuchukwu, Leuko, Thorgersen, Nixon, Hawkins, Kelly, Adams: "Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 15, pp. 5840-5, 2013 (PubMed).

    de Dios, Turnbull, Barbour, Ontedhu, Ghannoum, Tissue: "Soil phosphorous and endogenous rhythms exert a larger impact than CO2 or temperature on nocturnal stomatal conductance in Eucalyptus tereticornis." in: Tree physiology, Vol. 33, Issue 11, pp. 1206-15, 2013 (PubMed).

    Frick, Asplin, Favus, Culbertson, Krieger, Bushinsky: "Increased biological response to 1,25(OH)(2)D(3) in genetic hypercalciuric stone-forming rats." in: American journal of physiology. Renal physiology, Vol. 304, Issue 6, pp. F718-26, 2013 (PubMed).

    Brignac-Huber, Reed, Backes: "Organization of NADPH-cytochrome P450 reductase and CYP1A2 in the endoplasmic reticulum--microdomain localization affects monooxygenase function." in: Molecular pharmacology, Vol. 79, Issue 3, pp. 549-57, 2011 (PubMed).

  • Target
    Phosphate
    Target Type
    Chemical
    Background
    Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use. The newly designed Phosphate Colorimetric Assay Kit provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around 650 nm. Phosphate concentrations between 1 μM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined. The Phosphate Colorimetric Assay Kit provides 500 assays using microtiter plates or 100 assays using 1 mL cuvettes.
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