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AMH ELISA Kit

AMH Reactivity: Human Colorimetric Competition ELISA 937.5 pg/mL - 15000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN414951
  • Target See all AMH ELISA Kits
    AMH (Anti-Mullerian Hormone (AMH))
    Reactivity
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    937.5 pg/mL - 15000 pg/mL
    Minimum Detection Limit
    937.5 pg/mL
    Application
    ELISA
    Purpose
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of AMH in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

    We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity

    This assay has high sensitivity and excellent specificity for detection of Anti-Mullerian Hormone (AMH).
    No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed.

    Cross-Reactivity (Details)
    No significant cross-reactivity or interference between Anti-Mullerian Hormone (AMH) and analogues was observed.
    Sensitivity
    354.2 pg/mL
    Components
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
    Featured
    Discover our best selling AMH ELISA Kit
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    Discover our top product AMH ELISA Kit
  • Application Notes
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    Comment

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Sample Volume
    50 μL
    Assay Time
    2 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards,
    2. Add 50μL standard or sample to each well.
      Then add 50μL prepared Detection Reagent A immediately.
      Shake and mix. Incubate 1 hour at 37 °C,
    3. Aspirate and wash 3 times,
    4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    5. Aspirate and wash 5 times,
    6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    7. Add 50μL Stop Solution. Read at 450 nm immediately.
    Reagent Preparation
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 0.5mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 15,000pg/mL. Prepare 5 tubes containing 0.25ml Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 15,000pg/mL, 7,500pg/mL, 3,750pg/mL, 1,875pg/mL, 937.5pg/mL, and the last tube with Standard Diluent is the blank as 0pg/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, kept for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the volume errors.
    4. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    5. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    7. Contaminated water or container for reagent preparation will influence the detection result.
    Assay Precision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Mullerian Hormone (AMH) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handling Advice
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Storage
    4 °C
    Storage Comment
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    Expiry Date
    6 months
  • El Shamy, Amer, Mohamed, James, Jayaprakasan: "The impact of uterine artery embolization on ovarian reserve: A systematic review and meta-analysis." in: Acta obstetricia et gynecologica Scandinavica, Vol. 99, Issue 1, pp. 16-23, (2020) (PubMed).

    Çelebi, Ordu, Ilgün, Oztürk, Erdoğan Iyigün, Alço, Duymaz, Aktepe, Soybir, Baysal, Özmen: "The Effect of Systemic Chemotherapy on Ovarian Function: A Prospective Clinical Trial." in: European journal of breast health, Vol. 16, Issue 3, pp. 177-182, (2020) (PubMed).

    Castillo-Martínez, Rivera, Mouneu-Ornelas, Martínez-Martínez, Jiménez-Rojas, Márquez-Velasco, Amezcua-Guerra: "Levels of anti-Müllerian hormone in premenopausal women with the antiphospholipid syndrome and its association with the risk of clinical complications." in: Lupus, Vol. 28, Issue 3, pp. 427-431, (2019) (PubMed).

    Czuczwar, Stepniak, Milart, Paszkowski, Wozniak: "Comparison of the influence of three fibroid treatment options: supracervical hysterectomy, ulipristal acetate and uterine artery embolization on ovarian reserve - an observational study." in: Journal of ovarian research, Vol. 11, Issue 1, pp. 45, (2018) (PubMed).

    Wu, Bersinger, Mueller, von Wolff: "Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis." in: Journal of assisted reproduction and genetics, Vol. 34, Issue 3, pp. 357-364, (2017) (PubMed).

    Kim, Seong, Kim, Kim, Choi, Kim, Park, Jin, Lee, Noh: "Prognostic Value of Anti-Müllerian Hormone and Inhibin B in Patients with Premenopausal Hormone Receptor-positive Breast Cancer." in: Anticancer research, Vol. 36, Issue 3, pp. 1051-7, (2016) (PubMed).

    Özcan, Fıçıcıoğlu, Kizilkale, Yesiladali, Tok, Ozkan, Esrefoglu: "Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage?" in: Journal of assisted reproduction and genetics, Vol. 33, Issue 9, pp. 1223-30, (2016) (PubMed).

    Kotanidis, Nikolettos, Petousis, Asimakopoulos, Chatzimitrou, Kolios, Nikolettos: "The use of serum anti-Mullerian hormone (AMH) levels and antral follicle count (AFC) to predict the number of oocytes collected and availability of embryos for cryopreservation in IVF." in: Journal of endocrinological investigation, Vol. 39, Issue 12, pp. 1459-1464, (2016) (PubMed).

    Yin, Tang, Chen, Li, Liu: "Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum." in: Environmental pollution (Barking, Essex : 1987), Vol. 220, Issue Pt B, pp. 1429-1437, (2016) (PubMed).

    Komarowska, Milewski, Charkiewicz, Matuszczak, Sulewska, Zelazowska-Rutkowska, Hermanowicz, Niklinski, Debek, Hermanowicz: "Are anti-Müllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes?" in: Advances in medical sciences, Vol. 61, Issue 2, pp. 288-292, (2016) (PubMed).

    Birdir, Fryze, Vasiliadis, Nicolaides, Poon: "Maternal serum anti-Müllerian hormone at 11-13 weeks' gestation in the prediction of preeclampsia." in: The journal of maternal-fetal & neonatal medicine, Vol. 28, Issue 8, pp. 865-8, (2015) (PubMed).

    Jung, Kim, Seo, Choi, Nam, Kim, Kim, Han, Kim, Kim: "Anti-Proliferative and Apoptotic Activities of Müllerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines." in: Yonsei medical journal, Vol. 57, Issue 1, pp. 33-40, (2015) (PubMed).

    Akar, Do?er, Çak?ro?lu, Çorapç?o?lu, Sarper, Çal??kan: "The effect of childhood cancer therapy on ovarian reserve and pubertal development." in: Reproductive biomedicine online, Vol. 30, Issue 2, pp. 175-80, (2015) (PubMed).

    Sikar Aktürk, Abalı, Yüksel, Güzel, Güzel, Kıran: "The effects of isotretinoin on the ovarian reserve of females with acne." in: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology, Vol. 30, Issue 1, pp. 30-3, (2014) (PubMed).

    Carrarelli, Rocha, Belmonte, Zupi, Abrão, Arcuri, Piomboni, Petraglia: "Increased expression of antimüllerian hormone and its receptor in endometriosis." in: Fertility and sterility, Vol. 101, Issue 5, pp. 1353-8, (2014) (PubMed).

    Koutlaki, Dimitraki, Zervoudis, Poiana, Psillaki, Nikas, Liberis, Badiu, Liberis: "The relationship between Anti-Müllerian hormone and other reproductive parameters in normal women and in women with polycystic ovary syndrome." in: Journal of medicine and life, Vol. 6, Issue 2, pp. 146-50, (2014) (PubMed).

    Kotanidis, Asimakopoulos, Nikolettos: "Association between AMH, oocyte number and availability of embryos for cryopreservation in IVF." in: In vivo (Athens, Greece), Vol. 27, Issue 6, pp. 877-80, (2013) (PubMed).

    Amer, Mahran, Abdelmaged, El-Adawy, Eissa, Shaw: "The influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: a pilot study." in: Reproductive biology and endocrinology : RB&E, Vol. 11, pp. 115, (2013) (PubMed).

    Abali, Yuksel, Aktas, Celik, Guzel, Erfan, Sahin: "Decreased ovarian reserve in female Sprague-Dawley rats induced by isotretinoin (retinoic acid) exposure." in: Reproductive biomedicine online, Vol. 27, Issue 2, pp. 184-91, (2013) (PubMed).

    Lin, Wang, Weng, Sheng, Jiang, Yang et al.: "Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu ..." in: Journal of Zhejiang University. Science. B, Vol. 13, Issue 11, pp. 894-903, (2012) (PubMed).

  • Target See all AMH ELISA Kits
    AMH (Anti-Mullerian Hormone (AMH))
    Alternative Name
    AMH (AMH Products)
    Synonyms
    AMH ELISA Kit, amh ELISA Kit, MIF ELISA Kit, MIS ELISA Kit, anti-Mullerian hormone ELISA Kit, amh ELISA Kit, AMH ELISA Kit, Amh ELISA Kit
    UniProt
    P03971
    Pathways
    Negative Regulation of Hormone Secretion
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