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Anti-PAR2 Antibody (IgG) ELISA Kit

Colorimetric Competition ELISA
Catalog No. ABIN454019
  • Target
    Anti-PAR2 Antibody (IgG)
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Application
    ELISA
    Purpose
    The anti-alpha2-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the Alpha2-adrenergic-receptor in serum and plasma.
    Analytical Method
    Quantitative
    Characteristics
    The anti-Thrombin-Receptor-like-1-Antibody-EIA is an antibody screening test.
  • Plate
    Pre-coated
    Protocol
    The Thrombin-Receptor-like-1 has been pre-coated onto a microtiter plate. During the first incubation the anti-Thrombin-Receptor-like-1-Antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-Thrombin-Receptor-like-1-Antibody.
    Assay Procedure

    Incubation of samples/ standards/ controls: 100 µL 120 min, 4°C
    Wash Incubation of detection antibody: 100 µL 1 hr, room temperature
    Wash Substrate incubation: 100 µL/well 20 min, room temperature
    Add Stopp solution: 100 µL/well
    Read at 450nm

    Assay Precision
    Intraassay-Precision:7,83% Interassay-Precision:9,12%
    Restrictions
    For Research Use only
  • Storage
    4 °C
  • Target
    Anti-PAR2 Antibody (IgG)
    Target Type
    Antibody
    Background
    The Alpha-2 adrenergic receptor is a G protein-coupled receptor. ß receptors have the subtypes ß1, ß2 and ß3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP.
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