IgM ELISA Kit
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- Target See all IgM ELISA Kits
- IgM
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Reactivity
- Rabbit
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Minimum Detection Limit
- 782 ng/mL
- Application
- ELISA
- Purpose
- The IgM(rabbit)-ELISA is designed for the quantitative determination of rabbit IgM in complex samples (serum or other biological samples).
- Sample Type
- Serum
- Analytical Method
- Quantitative
- Characteristics
- The determination of rabbit IgM is carried out as direct sandwich ELISA.
- Featured
- Discover our best selling IgM ELISA Kit
- Top Product
- Discover our top product IgM ELISA Kit
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IgM ELISA Kit
Reactivity: Chicken Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
IgM ELISA KitReactivity: Human Colorimetric Sandwich ELISA 31.2 ng/mL - 2000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Grouper (Epinephelinae) Colorimetric Competition ELISA 1.25-50 μg/mL Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 25 ng/mL - 800 ng/mL Plasma, Serum, Urine
IgM ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 31.25 ng/mL - 1000 ng/mL Plasma, Serum, Urine
IgM ELISA KitReactivity: Human Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Plasma, Serum, Urine
IgM ELISA KitReactivity: Dog Colorimetric Sandwich ELISA 25 ng/mL - 800 ng/mL Plasma, Serum, Urine
IgM ELISA KitReactivity: Cow Colorimetric Competition ELISA 0.39 μg/mL - 25 μg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
IgM ELISA KitReactivity: Chicken Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Urine
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- Plate
- Pre-coated
- Protocol
- An antibody specific for rabbit IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgM present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgM concentration.
- Assay Procedure
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm - Assay Precision
- Intraassay precision (CV):(n=10)at 42.0 ng/ml: 3.1%at 176.9 ng/ml: 2.3%at 382.1 ng/ml: 5.5%
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all IgM ELISA Kits
- IgM
- Abstract
- IgM Products
- Synonyms
- IgM constant region ELISA Kit, IGM ELISA Kit
- Target Type
- Antibody
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