Cholecystokinin ELISA Kit

Catalog No. ABIN454698

Product Details Cholecystokinin ELISA Kit

Target: Cholecystokinin (CCK)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Mouse Cholecystokinin,CCK, concentrations in serum, tissue homogenates and other biological fluids.

Sample Type: Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse CCK.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Mus musculus,Mouse,Cholecystokinin,CCK,Cck

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CCK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CCK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CCK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for Cholecystokinin

Target: Cholecystokinin (CCK)

Alternative Name: Cck (CCK Products)

Synonyms: CCK ELISA Kit, CCK-CH ELISA Kit, cck-a ELISA Kit, cck-b ELISA Kit, cholecystokinin ELISA Kit, cholecystokinin L homeolog ELISA Kit, CCK ELISA Kit, Cck ELISA Kit, cck ELISA Kit, cck.L ELISA Kit

Pathways: TCR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades, Feeding Behaviour