COL1A1 ELISA Kit

Catalog No. ABIN454771

Product Details COL1A1 ELISA Kit

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Purpose: This immunoassay kit allows for the specific measurement of mouse Collagen Type Ⅰ concentrations in cell culture supernates, serum, and plasma.

Sample Type: Cell Culture Supernatant, Serum, Plasma

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse Collagen Type Ⅰ.

Characteristics: Mus musculus,Mouse,Collagen alpha-1(I) chain,Alpha-1 type I collagen,Col1a1,Cola1

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Collagen Type Ⅰ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Collagen Type Ⅰ present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Collagen Type Ⅰ is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Collagen Type Ⅰ bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for COL1A1

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Alternative Name: Col1a1 (COL1A1 Products)

Synonyms: OI4 ELISA Kit, Col1a-1 ELISA Kit, Cola-1 ELISA Kit, Cola1 ELISA Kit, Mov-13 ELISA Kit, Mov13 ELISA Kit, COLIA1 ELISA Kit, col1a1 ELISA Kit, MGC52532 ELISA Kit, COL1A1 ELISA Kit, COL1A2 ELISA Kit, alpha1(I) ELISA Kit, cb21 ELISA Kit, sb:cb21 ELISA Kit, sb:cb384 ELISA Kit, wu:fa99c12 ELISA Kit, wu:fb02c06 ELISA Kit, alpha3(I) ELISA Kit, col1a3 ELISA Kit, hm:zeh0348 ELISA Kit, wu:fa95h05 ELISA Kit, wu:fd02a10 ELISA Kit, wu:fj59a10 ELISA Kit, collagen type I alpha 1 chain ELISA Kit, collagen, type I, alpha 1 ELISA Kit, collagen, type I, alpha 1 S homeolog ELISA Kit, collagen alpha-1(I) chain ELISA Kit, collagen type I alpha 2 chain ELISA Kit, collagen, type I, alpha 1a ELISA Kit, collagen 1a1 ELISA Kit, collagen, type I, alpha 1b ELISA Kit, COL1A1 ELISA Kit, Col1a1 ELISA Kit, col1a1.S ELISA Kit, LOC397571 ELISA Kit, COL1A2 ELISA Kit, col1a1a ELISA Kit, col1a1 ELISA Kit, col1a1b ELISA Kit

Background: Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Type 1 collagen is the most abundant protein in the connective tissues, especially in tendon, skin and bone.

Pathways: Sensory Perception of Sound, Autophagy, Growth Factor Binding