This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for KIM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KIM-1 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for KIM-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions
For Research Use only
Storage
4 °C/-20 °C
Storage Comment
The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
Gil, Yang, Lee, Hong: "Clinical implication of urinary neutrophil gelatinase-associated lipocalin and kidney injury molecule-1 in patients with acute paraquat intoxication." in: Clinical toxicology (Philadelphia, Pa.), Vol. 47, Issue 9, pp. 870-5, (2009) (PubMed).
Kidney injury molecule-1 (KIM-1) is a type 1 transmembrane protein that is not detectable in normal kidney tissue but is expressed at high levels in rodent kidneys with dedifferentiated proximal tubule epithelial cells after ischemic or toxic injury.