Bisphenol A ELISA Kit
Reactivity: Chemical
Colorimetric
Competition ELISA
0.01-1 μg/mL
Soil, Water
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- Target
- Bisphenol A
- Reactivity
- Chemical
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Detection Range
- 0.01-1 μg/mL
- Minimum Detection Limit
- 0.01 μg/mL
- Application
- ELISA
- Purpose
- ELISA-VIDITEST Bisphenol A is based on the competition of BPA present in the sample and BPA immobilized to the wells of the microtitrate plate for binding sites of the anti-BPA polyclonal chicken antibody.
- Sample Type
- Soil, Water
- Analytical Method
- Quantitative
- Sensitivity
- 10 ng/mL
- Characteristics
- ELISA-VIDITEST Bisphenol A is intended for the detection of bisphenol A (BPA) in environmental samples. The microtitrate plate with 96 test wells allows measuring a large set of samples simultaneously. The bound antibodies are detected with anti-chicken antibody peroxidase conjugate anti-IgY Px. BPA present in the sample is determined by a color reaction with the chromogenic substrate (TMB). If the samples contain low concentrations of BPA, more molecules of anti-BPA antibody are available for binding to the immobilized BPA and the resulting colour of the wells is dark yellow. If the samples contain high concentrations of BPA, less molecules of the anti-BPA antibody bind to the immobilized BPA and the colour of wells is light yellow-white. The colour development is inversely proportional to the BPA concentration in the sample. The standard curve is constructed by plotting the absorbance of standards at 450 nm (Y-axis) versus log of the BPA concentrations in μg/mL present in the standards (X-axis). The unknown BPA concentration in the samples is calculated from the standard curve.
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- Comment
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Origin of standards: bisphenol A
Number of standards: 6 - Assay Time
- 2.5 h
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Target
- Bisphenol A
- Target Type
- Chemical
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