Aggrecan ELISA Kit
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- Target See all Aggrecan (ACAN) ELISA Kits
- Aggrecan (ACAN)
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 41-10.000 pg/mL
- Minimum Detection Limit
- 41 pg/mL
- Application
- ELISA
- Purpose
- Human Aggrecan ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Sample Type
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair recognizes Human Aggrecan.
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product ACAN ELISA Kit
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- Application Notes
- Recommended Dilution for serum and plasma samples2 fold
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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- Target See all Aggrecan (ACAN) ELISA Kits
- Aggrecan (ACAN)
- Alternative Name
- Aggrecan (ACAN Products)
- Synonyms
- AGC1 ELISA Kit, AGCAN ELISA Kit, CSPG1 ELISA Kit, CSPGCP ELISA Kit, MSK16 ELISA Kit, SEDK ELISA Kit, Agc ELISA Kit, Agc1 ELISA Kit, Cspg1 ELISA Kit, b2b183Clo ELISA Kit, cmd ELISA Kit, aggrecan ELISA Kit, aggrecan core protein ELISA Kit, ACAN ELISA Kit, Acan ELISA Kit, LOC100380783 ELISA Kit, AGC ELISA Kit
- UniProt
- P16112
- Pathways
- Glycosaminoglycan Metabolic Process, Dicarboxylic Acid Transport
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