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AKR1C3 ELISA Kit
AKR1C3
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details AKR1C3 ELISA Kit
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Target
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AKR1C3
(Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human AKR1C3 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Human BIRC4.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for AKR1C3
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Target
See all AKR1C3 ELISA Kits
AKR1C3
(Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))
Alternative Name
AKR1C3 (AKR1C3 Products )
Synonyms
AKR1C3 ELISA Kit, PGFS ELISA Kit, MGC134333 ELISA Kit, DD3 ELISA Kit, DDX ELISA Kit, HA1753 ELISA Kit, HAKRB ELISA Kit, HAKRe ELISA Kit, HSD17B5 ELISA Kit, hluPGFS ELISA Kit, Akr1c18 ELISA Kit, aldo-keto reductase family 1, member C3 L homeolog ELISA Kit, aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II) ELISA Kit, aldo-keto reductase family 1 member C2 ELISA Kit, aldo-keto reductase family 1 member C3 ELISA Kit, aldo-keto reductase family 1, member C3 ELISA Kit, akr1c3.L ELISA Kit, AKR1C3 ELISA Kit, LOC722496 ELISA Kit, Akr1c3 ELISA Kit
Gene ID
8644
UniProt
P42330
Pathways
Retinoic Acid Receptor Signaling Pathway , Steroid Hormone Biosynthesis , Regulation of Hormone Metabolic Process , Regulation of Hormone Biosynthetic Process , C21-Steroid Hormone Metabolic Process , Protein targeting to Nucleus
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