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AKR1C3 ELISA Kit

AKR1C3 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4881786
  • Target See all AKR1C3 ELISA Kits
    AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human AKR1C3 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human BIRC4.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all AKR1C3 ELISA Kits
    AKR1C3 (Aldo-Keto Reductase Family 1, Member C3 (3-alpha Hydroxysteroid Dehydrogenase, Type II) (AKR1C3))
    Alternative Name
    AKR1C3 (AKR1C3 Products)
    Synonyms
    AKR1C3 ELISA Kit, PGFS ELISA Kit, MGC134333 ELISA Kit, DD3 ELISA Kit, DDX ELISA Kit, HA1753 ELISA Kit, HAKRB ELISA Kit, HAKRe ELISA Kit, HSD17B5 ELISA Kit, hluPGFS ELISA Kit, Akr1c18 ELISA Kit, aldo-keto reductase family 1, member C3 L homeolog ELISA Kit, aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II) ELISA Kit, aldo-keto reductase family 1 member C2 ELISA Kit, aldo-keto reductase family 1 member C3 ELISA Kit, aldo-keto reductase family 1, member C3 ELISA Kit, akr1c3.L ELISA Kit, AKR1C3 ELISA Kit, LOC722496 ELISA Kit, Akr1c3 ELISA Kit
    Gene ID
    8644
    UniProt
    P42330
    Pathways
    Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, C21-Steroid Hormone Metabolic Process, Protein targeting to Nucleus
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