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B3GNT2 ELISA Kit

B3GNT2 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4881916
  • Target See all B3GNT2 products
    B3GNT2 (UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human B3GNT2 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all B3GNT2 products
    B3GNT2 (UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))
    Alternative Name
    B3GNT2 (B3GNT2 Products)
    Synonyms
    B3GN-T2 ELISA Kit, B3GNT ELISA Kit, B3GNT-2 ELISA Kit, B3GNT1 ELISA Kit, BETA3GNT ELISA Kit, BGNT2 ELISA Kit, BGnT-2 ELISA Kit, AA408337 ELISA Kit, AA409404 ELISA Kit, AW260308 ELISA Kit, B3Galt6 ELISA Kit, B3gnt1 ELISA Kit, BGnT-1 ELISA Kit, BGnT1 ELISA Kit, BGnT2 ELISA Kit, Beta3gnt ELISA Kit, MGC81185 ELISA Kit, B3GNT2 ELISA Kit, MGC179257 ELISA Kit, ssp5 ELISA Kit, wu:fa55h04 ELISA Kit, wu:fc39c01 ELISA Kit, zgc:113947 ELISA Kit, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 ELISA Kit, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 L homeolog ELISA Kit, beta-1,4-glucuronyltransferase 1 ELISA Kit, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2b ELISA Kit, B3GNT2 ELISA Kit, B3gnt2 ELISA Kit, b3gnt2.L ELISA Kit, B4GAT1 ELISA Kit, b3gnt2b ELISA Kit, b3gnt2 ELISA Kit
    Gene ID
    10678
    UniProt
    Q9NY97
    Pathways
    Glycosaminoglycan Metabolic Process
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