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Calreticulin 3 ELISA Kit

CALR3 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4882077
  • Target See all Calreticulin 3 (CALR3) ELISA Kits
    Calreticulin 3 (CALR3)
    Reactivity
    • 2
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human Calreticulin-2 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human ENPP-5.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all Calreticulin 3 (CALR3) ELISA Kits
    Calreticulin 3 (CALR3)
    Alternative Name
    Calreticulin-2 (CALR3 Products)
    Synonyms
    1700031L01Rik ELISA Kit, 6330586I20Rik ELISA Kit, Crt2 ELISA Kit, cspn ELISA Kit, CMH19 ELISA Kit, CRT2 ELISA Kit, CT93 ELISA Kit, A. thaliana calreticulin 3 ELISA Kit, AtCRT3 ELISA Kit, CALRETICULIN 3 ELISA Kit, EBS2 ELISA Kit, EMS-MUTAGENIZED BRI1 SUPPRESSOR 2 ELISA Kit, PRIORITY IN SWEET LIFE 1 ELISA Kit, PSL1 ELISA Kit, T27G7.13 ELISA Kit, T27G7_13 ELISA Kit, calreticulin 3 ELISA Kit, CALR ELISA Kit, calreticulin 3 ELISA Kit, CALR3 ELISA Kit, Calr3 ELISA Kit, CRT3 ELISA Kit
    Gene ID
    125972
    UniProt
    Q96L12
    Pathways
    Activation of Innate immune Response
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