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DDX11 ELISA Kit

DDX11 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4882333
  • Target See all DDX11 ELISA Kits
    DDX11 (DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11))
    Reactivity
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    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human Cell Adhesion Molecule with Homology to L1CAM (L1CAM-2) ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Human Cell Adhesion Molecule with Homology to L1CAM (L1CAM-2)
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all DDX11 ELISA Kits
    DDX11 (DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11))
    Alternative Name
    CHL-1 (DDX11 Products)
    Synonyms
    CHL1 ELISA Kit, CHLR1 ELISA Kit, KRG2 ELISA Kit, WABS ELISA Kit, 4732462I11Rik ELISA Kit, essa15a ELISA Kit, putative ATP-dependent RNA helicase DDX11-like protein 8 ELISA Kit, DEAD/H-box helicase 11 ELISA Kit, DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 ELISA Kit, LOC100448472 ELISA Kit, DDX11 ELISA Kit, Ddx11 ELISA Kit
    Gene ID
    10752
    UniProt
    O00533
    Pathways
    ER-Nucleus Signaling
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