Cytokeratin 18 ELISA Kit

Catalog No. ABIN4882548

Product Details Cytokeratin 18 ELISA Kit

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.164-40 ng/mL

Minimum Detection Limit: 0.164 ng/mL

Application: ELISA

Purpose: Human Cytokeratin 18/KRT18 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Cytokeratin 18.

Sensitivity: 160 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Cytokeratin 18 (KRT18 Products)

Synonyms: CK18 ELISA Kit, K18 ELISA Kit, Krt1-18 ELISA Kit, CYK18 ELISA Kit, DreK18 ELISA Kit, cb83 ELISA Kit, dapk1 ELISA Kit, sb:cb83 ELISA Kit, wu:fa13f02 ELISA Kit, wu:fb36b04 ELISA Kit, krt18 ELISA Kit, MGC64569 ELISA Kit, MGC75922 ELISA Kit, KRT18 ELISA Kit, CK-18-A ELISA Kit, K18-A ELISA Kit, NC-11 ELISA Kit, cyk18 ELISA Kit, k18 ELISA Kit, krt18-a ELISA Kit, krt18-b ELISA Kit, krt18a ELISA Kit, xkendob ELISA Kit, keratin 18 ELISA Kit, keratin 18, type I L homeolog ELISA Kit, keratin 18, type I ELISA Kit, keratin, type I cytoskeletal 18 ELISA Kit, K18, simple type I keratin ELISA Kit, Keratin, type I cytoskeletal 18 ELISA Kit, keratin 18, type I S homeolog ELISA Kit, Krt18 ELISA Kit, KRT18 ELISA Kit, krt18 ELISA Kit, krt18.L ELISA Kit, LOC700569 ELISA Kit, LOC100136778 ELISA Kit, k1c18 ELISA Kit, LOC100008885 ELISA Kit, krt18.S ELISA Kit

Gene ID: 3875

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis