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DDR1 ELISA Kit

DDR1 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4882586
  • Target See all DDR1 ELISA Kits
    DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))
    Reactivity
    • 6
    • 2
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human DDR1 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all DDR1 ELISA Kits
    DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))
    Alternative Name
    DDR1 (DDR1 Products)
    Synonyms
    CG11573 ELISA Kit, CG13984 ELISA Kit, CG13985 ELISA Kit, CG33531 ELISA Kit, CG9488 ELISA Kit, CG9490 ELISA Kit, CT33540 ELISA Kit, DDR ELISA Kit, Dmel\\CG33531 ELISA Kit, Dmel_CG13984 ELISA Kit, 6030432F18 ELISA Kit, AI323681 ELISA Kit, CD167a ELISA Kit, Cak ELISA Kit, Nep ELISA Kit, PTK3A ELISA Kit, CAK ELISA Kit, CD167 ELISA Kit, EDDR1 ELISA Kit, HGK2 ELISA Kit, MCK10 ELISA Kit, NEP ELISA Kit, NTRK4 ELISA Kit, PTK3 ELISA Kit, RTK6 ELISA Kit, TRKE ELISA Kit, Drd1 ELISA Kit, PTK3D ELISA Kit, Discoidin domain receptor ELISA Kit, discoidin domain receptor family, member 1 ELISA Kit, discoidin domain receptor tyrosine kinase 1 ELISA Kit, Ddr ELISA Kit, Ddr1 ELISA Kit, DDR1 ELISA Kit
    Gene ID
    780
    UniProt
    Q08345
    Pathways
    RTK Signaling, Smooth Muscle Cell Migration
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