S100A12 ELISA Kit
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- Target See all S100A12 ELISA Kits
- S100A12 (S100 Calcium Binding Protein A12 (S100A12))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.82-200 ng/mL
- Minimum Detection Limit
- 0.82 ng/mL
- Application
- ELISA
- Purpose
- Human EN-RAGE (S100 A12) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- Sample Type
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair recognizes Human EN-RAGE. Other species not yet determined.
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Application Notes
- Recommended Dilution for serum and plasma samples2 fold
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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- Target See all S100A12 ELISA Kits
- S100A12 (S100 Calcium Binding Protein A12 (S100A12))
- Alternative Name
- EN-RAGE (S100A12 Products)
- Synonyms
- CAAF1 ELISA Kit, CAGC ELISA Kit, CGRP ELISA Kit, ENRAGE ELISA Kit, MRP-6 ELISA Kit, MRP6 ELISA Kit, p6 ELISA Kit, CO-AG ELISA Kit, EN-RAGE ELISA Kit, S100 calcium binding protein A12 ELISA Kit, protein S100-A12 ELISA Kit, S100A12 ELISA Kit, LOC101103771 ELISA Kit
- Gene ID
- 6283
- UniProt
- P80511
- Pathways
- Toll-Like Receptors Cascades, S100 Proteins
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