FABP4 ELISA Kit

Catalog No. ABIN4882788

Product Details FABP4 ELISA Kit

Target: FABP4 (Fatty Acid Binding Protein 4, Adipocyte (FABP4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human FABP4/A-FABP ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human FABP4.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for FABP4

Target: FABP4 (Fatty Acid Binding Protein 4, Adipocyte (FABP4))

Alternative Name: FABP4 (FABP4 Products)

Synonyms: A-FABP ELISA Kit, AFABP ELISA Kit, ALBP ELISA Kit, aP2 ELISA Kit, LOC100223994 ELISA Kit, FABP ELISA Kit, AP2 ELISA Kit, FABP3 ELISA Kit, FABP4 ELISA Kit, MGC84940 ELISA Kit, fabp4 ELISA Kit, 422/aP2 ELISA Kit, ALBP/Ap2 ELISA Kit, Ap2 ELISA Kit, Lbpl ELISA Kit, Albp ELISA Kit, fatty acid binding protein 4 ELISA Kit, fatty acid-binding protein, adipocyte ELISA Kit, fatty acid binding protein 4, adipocyte ELISA Kit, fatty acid binding protein 4, adipocyte L homeolog ELISA Kit, Fatty acid-binding protein, adipocyte ELISA Kit, adipocyte fatty acid-binding protein ELISA Kit, adipocyte fatty acid binding protein ELISA Kit, FABP4 ELISA Kit, LOC100223994 ELISA Kit, fabp4.L ELISA Kit, fabp4 ELISA Kit, Fabp4 ELISA Kit, LOC100732353 ELISA Kit, LOC100861279 ELISA Kit, LOC100057425 ELISA Kit, LOC395224 ELISA Kit

Gene ID: 2167

UniProt: P15090

Pathways: Brown Fat Cell Differentiation