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FCGR3B ELISA Kit
FCGR3B
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details FCGR3B ELISA Kit
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Target
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FCGR3B
(Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B))
Reactivity
All reactivities for FCGR3B elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human Fc gamma RIIIB (CD16b) ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for FCGR3B
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Target
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FCGR3B
(Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B))
Alternative Name
Fc gamma RIIIB (FCGR3B Products )
Synonyms
CD16 ELISA Kit, CD16b ELISA Kit, FCG3 ELISA Kit, FCGR3 ELISA Kit, FCR-10 ELISA Kit, FCRIII ELISA Kit, FCRIIIb ELISA Kit, FcRIII ELISA Kit, Fc fragment of IgG receptor IIIb ELISA Kit, Fc receptor, IgG, low affinity III ELISA Kit, Fc fragment of IgG receptor IIIa ELISA Kit, Fc-gamma RIII ELISA Kit, FCGR3B ELISA Kit, Fcgr3 ELISA Kit, FCGR3A ELISA Kit, FCGR3 ELISA Kit
Gene ID
2215
UniProt
O75015
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