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GLI3 ELISA Kit

GLI3 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4883081
  • Target See all GLI3 ELISA Kits
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human GLI-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human GLI-3.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all GLI3 ELISA Kits
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    Alternative Name
    GLI-3 (GLI3 Products)
    Synonyms
    ACLS ELISA Kit, GCPS ELISA Kit, GLI3-190 ELISA Kit, GLI3FL ELISA Kit, PAP-A ELISA Kit, PAPA ELISA Kit, PAPA1 ELISA Kit, PAPB ELISA Kit, PHS ELISA Kit, PPDIV ELISA Kit, xgli3 ELISA Kit, AI854843 ELISA Kit, AU023367 ELISA Kit, Bph ELISA Kit, Pdn ELISA Kit, Xt ELISA Kit, add ELISA Kit, im:7142603 ELISA Kit, GLI3 ELISA Kit, GLI family zinc finger 3 ELISA Kit, GLI family zinc finger 3 L homeolog ELISA Kit, GLI-Kruppel family member GLI3 ELISA Kit, GLI3 ELISA Kit, Gli3 ELISA Kit, gli3.L ELISA Kit, gli3 ELISA Kit
    Gene ID
    2737
    UniProt
    P10071
    Pathways
    Hedgehog Signaling
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