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IDUA ELISA Kit

IDUA Reactivity: Human Colorimetric Sandwich ELISA 0.16-40 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4883259
  • Target See all IDUA ELISA Kits
    IDUA (Iduronidase, alpha-L- (IDUA))
    Reactivity
    • 4
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.16-40 ng/mL
    Minimum Detection Limit
    0.16 ng/mL
    Application
    ELISA
    Purpose
    Human IDUA ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair detects human IDUA. Other species not determined.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all IDUA ELISA Kits
    IDUA (Iduronidase, alpha-L- (IDUA))
    Alternative Name
    IDUA (IDUA Products)
    Synonyms
    IDA ELISA Kit, MPS1 ELISA Kit, 6030426D08 ELISA Kit, alpha-L-iduronidase ELISA Kit, MGC80842 ELISA Kit, si:ch211-12e13.9 ELISA Kit, IDUA ELISA Kit, iduronidase, alpha-L- ELISA Kit, iduronidase, alpha-L- L homeolog ELISA Kit, alpha-L-iduronidase ELISA Kit, IDUA ELISA Kit, Idua ELISA Kit, idua.L ELISA Kit, idua ELISA Kit, LOC5564727 ELISA Kit
    Gene ID
    3425
    UniProt
    P35475
    Pathways
    Glycosaminoglycan Metabolic Process
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