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IFNAR2 ELISA Kit

IFNAR2 Reactivity: Human Colorimetric Sandwich ELISA 0.16-40 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4883265
  • Target See all IFNAR2 ELISA Kits
    IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))
    Reactivity
    • 4
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.16-40 ng/mL
    Minimum Detection Limit
    0.16 ng/mL
    Application
    ELISA
    Purpose
    Human IFN-alpha / beta R2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM9, CD244, APRIL, BMP-2, BMP-9, Cathepsin L, CD200, CD97, Chemerin, C5a, DcR3, FABP2, FAP, FGF-19, Galectin-3, HGFR, FABP2, FAP, FGF-19, IFG-2R, IL-1R6, IL-24, IL-33, Kallikrein 14, Legumain, LOX-1, MBL, Neprilysin, Notch-1, NOV, Osteoactivin, PG-1, PGRPs, SerpinA4, sFRP-3, Thrombomodulin, TLR2, Transferrin, WIF-1
    Sensitivity
    0.16 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all IFNAR2 ELISA Kits
    IFNAR2 (Interferon alpha/beta Receptor 2 (IFNAR2))
    Alternative Name
    IFN alpha/beta R2 (IFNAR2 Products)
    Synonyms
    AI747302 ELISA Kit, Ifnar-2 ELISA Kit, IFN-R ELISA Kit, IFN-alpha-REC ELISA Kit, IFNABR ELISA Kit, IFNARB ELISA Kit, IFN-R-2 ELISA Kit, IFNBR ELISA Kit, interferon alpha and beta receptor subunit 2 ELISA Kit, interferon (alpha and beta) receptor 2 ELISA Kit, IFNAR2 ELISA Kit, Ifnar2 ELISA Kit
    Background
    Gene Names: IFNAR2 IFNABR IFNARB
    Protein names: Interferon alpha/beta receptor 2 (IFN-R-2) (IFN-alpha binding protein) (IFN-alpha/beta receptor 2) (Interferon alpha binding protein) (Type I interferon receptor 2)
    Gene ID
    3455
    UniProt
    P48551
    Pathways
    JAK-STAT Signaling, Hepatitis C
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