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JAM2 ELISA Kit

JAM2 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4883497
  • Target See all JAM2 ELISA Kits
    JAM2 (Junctional Adhesion Molecule 2 (JAM2))
    Reactivity
    • 3
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human Junctional Adhesion Molecule B (JAM2 / VE-JAM / CD322) ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Human Junctional Adhesion Molecule B (JAM2 / VE-JAM / CD322)
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all JAM2 ELISA Kits
    JAM2 (Junctional Adhesion Molecule 2 (JAM2))
    Alternative Name
    JAM-B (JAM2 Products)
    Synonyms
    JAM2 ELISA Kit, cd322 ELISA Kit, jam-b ELISA Kit, jamb ELISA Kit, pro245 ELISA Kit, ve-jam ELISA Kit, vejam ELISA Kit, jam2 ELISA Kit, sb:cb1002 ELISA Kit, im:7155552 ELISA Kit, sc:d0813 ELISA Kit, si:ch211-160b11.5 ELISA Kit, LOC100227766 ELISA Kit, 1110002N23Rik ELISA Kit, 2410030G21Rik ELISA Kit, 2410167M24Rik ELISA Kit, JAM-2 ELISA Kit, JAM-B ELISA Kit, Jcam2 ELISA Kit, VE-JAM ELISA Kit, C21orf43 ELISA Kit, CD322 ELISA Kit, JAMB ELISA Kit, PRO245 ELISA Kit, VEJAM ELISA Kit, junctional adhesion molecule 2 ELISA Kit, junctional adhesion molecule 2a ELISA Kit, junctional adhesion molecule 2b ELISA Kit, junction adhesion molecule 2 ELISA Kit, JAM2 ELISA Kit, jam2 ELISA Kit, jam2a ELISA Kit, jam2b ELISA Kit, Jam2 ELISA Kit
    Gene ID
    58494
    UniProt
    P57087
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