KRT8 ELISA Kit

Catalog No. ABIN4883555

Product Details KRT8 ELISA Kit

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.410-100 pg/mL

Minimum Detection Limit: 0.410 pg/mL

Application: ELISA

Purpose: Human Cytokeratin 8/KRT8 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Cytokeratin 8.

Sensitivity: 0.4 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8 (KRT8 Products)

Synonyms: CARD2 ELISA Kit, CK-8 ELISA Kit, CK8 ELISA Kit, CYK8 ELISA Kit, K2C8 ELISA Kit, K8 ELISA Kit, KO ELISA Kit, AA960620 ELISA Kit, AL022697 ELISA Kit, AU019895 ELISA Kit, Card2 ELISA Kit, EndoA ELISA Kit, Krt-2.8 ELISA Kit, Krt2-8 ELISA Kit, CYKER ELISA Kit, KRT2-8 ELISA Kit, KERATIN8 ELISA Kit, ck8 ELISA Kit, cyk8 ELISA Kit, k2c8 ELISA Kit, card2 ELISA Kit, krt2-5 ELISA Kit, MGC69490 ELISA Kit, KRT8 ELISA Kit, DreK8 ELISA Kit, cb186 ELISA Kit, krt2-8 ELISA Kit, sb:cb186 ELISA Kit, wu:fa20h05 ELISA Kit, wu:fa95h10 ELISA Kit, wu:fb96c06 ELISA Kit, zf-K8 ELISA Kit, zfk8 ELISA Kit, DKFZp468F2127 ELISA Kit, keratin 8 ELISA Kit, keratin 8 S homeolog ELISA Kit, KRT8 ELISA Kit, Krt8 ELISA Kit, krt8.S ELISA Kit, krt8 ELISA Kit

Gene ID: 3856

UniProt: P05787