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SCARB2 ELISA Kit

SCARB2 Reactivity: Human Colorimetric Sandwich ELISA 10-2500 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4883617
  • Target See all SCARB2 ELISA Kits
    SCARB2 (Scavenger Receptor Class B, Member 2 (SCARB2))
    Reactivity
    • 2
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    10-2500 pg/mL
    Minimum Detection Limit
    10 pg/mL
    Application
    ELISA
    Purpose
    Human LIMPII ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    Sensitivity
    10 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all SCARB2 ELISA Kits
    SCARB2 (Scavenger Receptor Class B, Member 2 (SCARB2))
    Alternative Name
    LIMP-II (SCARB2 Products)
    Synonyms
    cd36l2 ELISA Kit, fi13c07 ELISA Kit, chunp6914 ELISA Kit, wu:fi13c07 ELISA Kit, SCARB2 ELISA Kit, scarb2 ELISA Kit, AMRF ELISA Kit, CD36L2 ELISA Kit, EPM4 ELISA Kit, HLGP85 ELISA Kit, LGP85 ELISA Kit, LIMP-2 ELISA Kit, LIMPII ELISA Kit, SR-BII ELISA Kit, 9330185J12Rik ELISA Kit, Cd36l2 ELISA Kit, MLGP85 ELISA Kit, LimpII ELISA Kit, scavenger receptor class B, member 2a ELISA Kit, scavenger receptor class B member 2 ELISA Kit, scavenger receptor class B, member 2 ELISA Kit, scarb2a ELISA Kit, SCARB2 ELISA Kit, scarb2 ELISA Kit, Scarb2 ELISA Kit
    Background
    Gene Names: SCARB2 CD36L2 LIMP2 LIMPII
    Protein names: Lysosome membrane protein 2 (85 kDa lysosomal membrane sialoglycoprotein) (LGP85) (CD36 antigen-like 2) (Lysosome membrane protein II) (LIMP II) (Scavenger receptor class B member 2) (CD antigen CD36)
    Gene ID
    950
    UniProt
    Q14108
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