PCNA ELISA Kit

Catalog No. ABIN4884109

Product Details PCNA ELISA Kit

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2.5-600 ng/mL

Minimum Detection Limit: 2.5 ng/mL

Application: ELISA

Purpose: Human PCNA ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Human PCNA

Sensitivity: 2.46 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA (PCNA Products)

Synonyms: PCNAR ELISA Kit, Pcna/cyclin ELISA Kit, pcna ELISA Kit, AO090003000846 ELISA Kit, 53/13 ELISA Kit, CG9193 ELISA Kit, DmPCNA ELISA Kit, Dmel\\CG9193 ELISA Kit, MUS209 ELISA Kit, Mus209 ELISA Kit, PCNA ELISA Kit, PCNA/mus209 ELISA Kit, Pcna ELISA Kit, dPCNA ELISA Kit, l(2)02448 ELISA Kit, l(2)56F ELISA Kit, l(2)56Fa ELISA Kit, l(2)s1534 ELISA Kit, cb16 ELISA Kit, fb36g03 ELISA Kit, wu:fa28e03 ELISA Kit, wu:fb36g03 ELISA Kit, pcna-A ELISA Kit, proliferating cell nuclear antigen ELISA Kit, DNA polymerase III sliding clamp ELISA Kit, Proliferating cell nuclear antigen ELISA Kit, Proliferating cell nuclear antigen-like ELISA Kit, proliferating cell nuclear antigen L homeolog ELISA Kit, proliferating cell nuclear antigen 1 ELISA Kit, PCNA ELISA Kit, Pcna ELISA Kit, pcna ELISA Kit, MMP_RS08810 ELISA Kit, ANI_1_512014 ELISA Kit, AOR_1_1500154 ELISA Kit, MGC53867 ELISA Kit, pcna.L ELISA Kit, pcna1 ELISA Kit, LOC106420145 ELISA Kit, pcn1 ELISA Kit

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy