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PCSK2 ELISA Kit

PCSK2 Reactivity: Human Colorimetric Sandwich ELISA 2.458-600 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4884115
  • Target See all PCSK2 ELISA Kits
    PCSK2 (Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    2.458-600 ng/mL
    Minimum Detection Limit
    2.458 ng/mL
    Application
    ELISA
    Purpose
    Human PCSK2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human PCSK2.
    Sensitivity
    2.45 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all PCSK2 ELISA Kits
    PCSK2 (Proprotein Convertase Subtilisin/kexin Type 2 (PCSK2))
    Alternative Name
    PCSK2 (PCSK2 Products)
    Synonyms
    NEC 2 ELISA Kit, NEC-2 ELISA Kit, NEC2 ELISA Kit, PC2 ELISA Kit, SPC2 ELISA Kit, PCSK2 ELISA Kit, 6330411F23Rik ELISA Kit, AI839700 ELISA Kit, Nec-2 ELISA Kit, Nec2 ELISA Kit, Phpp-2 ELISA Kit, pc2 ELISA Kit, nec2 ELISA Kit, spc2 ELISA Kit, pcsk2 ELISA Kit, pcsk2-A ELISA Kit, proprotein convertase subtilisin/kexin type 2 ELISA Kit, proprotein convertase subtilisin/kexin type 2 L homeolog ELISA Kit, PCSK2 ELISA Kit, pcsk2 ELISA Kit, Pcsk2 ELISA Kit, pcsk2.L ELISA Kit
    Gene ID
    5126
    UniProt
    P16519
    Pathways
    Peptide Hormone Metabolism, cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity
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