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PLA2G4A ELISA Kit
PLA2G4A
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details PLA2G4A ELISA Kit
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Target
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PLA2G4A
(Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))
Reactivity
All reactivities for PLA2G4A elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human PLA2G4A ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes .
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternatives
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for PLA2G4A
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Target
See all PLA2G4A ELISA Kits
PLA2G4A
(Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))
Alternative Name
PLA2G4A (PLA2G4A Products )
Synonyms
PLA2G4 ELISA Kit, cPLA2-alpha ELISA Kit, Pla2c ELISA Kit, Pla2g4 ELISA Kit, cPLA2 ELISA Kit, pla2g4 ELISA Kit, CPLA2 ELISA Kit, PLA2G4A ELISA Kit, PLA2 ELISA Kit, PLA2G1B ELISA Kit, cPLA2alpha ELISA Kit, pla2g4a ELISA Kit, si:dkey-97o5.1 ELISA Kit, phospholipase A2 group IVA ELISA Kit, phospholipase A2 group IVA L homeolog ELISA Kit, phospholipase A2, group IVA (cytosolic, calcium-dependent) ELISA Kit, phospholipase A2, group IVAb (cytosolic, calcium-dependent) ELISA Kit, PLA2G4A ELISA Kit, Pla2g4a ELISA Kit, pla2g4a.L ELISA Kit, pla2g4ab ELISA Kit
Gene ID
5321
UniProt
P47712
Pathways
Inositol Metabolic Process , G-protein mediated Events , VEGF Signaling
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