Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

ROBO4 ELISA Kit

ROBO4 Reactivity: Human Colorimetric Sandwich ELISA 0.61-150 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4884371
  • Target See all ROBO4 ELISA Kits
    ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))
    Reactivity
    • 3
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.61-150 ng/mL
    Minimum Detection Limit
    0.61 ng/mL
    Application
    ELISA
    Purpose
    Human ROBO4 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes human ROBO4. Other species not yet determined.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling ROBO4 ELISA Kit
    Top Product
    Discover our top product ROBO4 ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all ROBO4 ELISA Kits
    ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))
    Alternative Name
    ROBO4 (ROBO4 Products)
    Synonyms
    ECSM4 ELISA Kit, MRB ELISA Kit, 1200012D01Rik ELISA Kit, AI593217 ELISA Kit, sc:d0823 ELISA Kit, wu:fa16f09 ELISA Kit, roundabout guidance receptor 4 ELISA Kit, roundabout, axon guidance receptor, homolog 4 (Drosophila) ELISA Kit, ROBO4 ELISA Kit, Robo4 ELISA Kit, robo4 ELISA Kit
    Gene ID
    54538
    UniProt
    Q8WZ75
You are here:
Support