S100A11 ELISA Kit

Catalog No. ABIN4884392

Product Details S100A11 ELISA Kit

Target: S100A11 (S100 Calcium Binding Protein A11 (S100A11))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.034-25 ng/mL

Minimum Detection Limit: 0.034 ng/mL

Application: ELISA

Purpose: Human S100 A11 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human S100 A11.

Sensitivity: 0.03 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for S100A11

Target: S100A11 (S100 Calcium Binding Protein A11 (S100A11))

Alternative Name: S100 A11 (S100A11 Products)

Synonyms: MLN70 ELISA Kit, S100C ELISA Kit, PCALG ELISA Kit, EMAPI ELISA Kit, Emap1 ELISA Kit, S100a14 ELISA Kit, S100c ELISA Kit, cal ELISA Kit, S100 calcium binding protein A11 ELISA Kit, S100A11 ELISA Kit, S100a11 ELISA Kit

Gene ID: 6282

UniProt: P31949

Pathways: S100 Proteins