SFRP1 ELISA Kit

Catalog No. ABIN4884499

Product Details SFRP1 ELISA Kit

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Purpose: Human sFRP-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human sFRP-1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples3 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for SFRP1

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Alternative Name: sFRP-1 (SFRP1 Products)

Synonyms: FRP ELISA Kit, FRP-1 ELISA Kit, FRP1 ELISA Kit, FrzA ELISA Kit, SARP2 ELISA Kit, 2210415K03Rik ELISA Kit, AW011917 ELISA Kit, AW107218 ELISA Kit, AW742929 ELISA Kit, sFRP-1 ELISA Kit, CSFRP1 ELISA Kit, SFRP1 ELISA Kit, XfrzA ELISA Kit, frp ELISA Kit, frp-1 ELISA Kit, frp1 ELISA Kit, frzA ELISA Kit, sarp2 ELISA Kit, sfrp1 ELISA Kit, LOC100231868 ELISA Kit, Srfp1a ELISA Kit, wu:fd19b09 ELISA Kit, secreted frizzled related protein 1 ELISA Kit, secreted frizzled-related protein 1 ELISA Kit, Secreted frizzled-related protein 1 ELISA Kit, secreted frizzled-related protein 1a ELISA Kit, secreted frizzled-related protein 1b ELISA Kit, SFRP1 ELISA Kit, Sfrp1 ELISA Kit, sfrp1 ELISA Kit, sfrp1a ELISA Kit, sfrp1b ELISA Kit

Gene ID: 6422

UniProt: Q8N474

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation