Sortilin 1 ELISA Kit

Catalog No. ABIN4884611

Product Details Sortilin 1 ELISA Kit

Target: Sortilin 1 (SORT1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.819-200 ng/mL

Minimum Detection Limit: 0.819 ng/mL

Application: ELISA

Purpose: Human Sortilin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Sortilin.

Sensitivity: 0.81 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Sortilin 1

Target: Sortilin 1 (SORT1)

Alternative Name: Sortilin (SORT1 Products)

Synonyms: SORT1 ELISA Kit, 19.m02079 ELISA Kit, gp95 ELISA Kit, ldlcq6 ELISA Kit, sortilin ELISA Kit, Gp95 ELISA Kit, LDLCQ6 ELISA Kit, NT3 ELISA Kit, 2900053A11Rik ELISA Kit, AI852375 ELISA Kit, Ntr3 ELISA Kit, Ntsr3 ELISA Kit, Nt3 ELISA Kit, Nts3 ELISA Kit, sort1 ELISA Kit, zgc:85953 ELISA Kit, sortilin 1 ELISA Kit, sortilin ELISA Kit, sortilin 1 S homeolog ELISA Kit, sortilin 1a ELISA Kit, SORT1 ELISA Kit, TP01_0016 ELISA Kit, BBOV_I002730 ELISA Kit, sort1.S ELISA Kit, Sort1 ELISA Kit, sort1a ELISA Kit

Gene ID: 6272

UniProt: Q99523

Pathways: Neurotrophin Signaling Pathway