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TLR4 ELISA Kit

TLR4 Reactivity: Human Colorimetric Sandwich ELISA 0.4-100 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4884827
  • Target See all TLR4 ELISA Kits
    TLR4 (Toll-Like Receptor 4 (TLR4))
    Reactivity
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.4-100 ng/mL
    Minimum Detection Limit
    0.4 ng/mL
    Application
    ELISA
    Purpose
    Human TLR4 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Human Toll-like Receptor 4 (TLR-4)
    Sensitivity
    0.4 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all TLR4 ELISA Kits
    TLR4 (Toll-Like Receptor 4 (TLR4))
    Alternative Name
    TLR-4 (TLR4 Products)
    Synonyms
    ARMD10 ELISA Kit, CD284 ELISA Kit, TLR-4 ELISA Kit, TOLL ELISA Kit, Lps ELISA Kit, Ly87 ELISA Kit, Ran/M1 ELISA Kit, Rasl2-8 ELISA Kit, toll like receptor 4 ELISA Kit, toll-like receptor 4 ELISA Kit, TLR4 ELISA Kit, Tlr4 ELISA Kit
    Background
    Gene Names: TLR4
    Protein names: Toll-like receptor 4 (hToll) (CD antigen CD284)
    Gene ID
    7099
    UniProt
    O00206
    Pathways
    TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins
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