TNFRSF1A ELISA Kit

Catalog No. ABIN4884842

Product Details TNFRSF1A ELISA Kit

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1-400 pg/mL

Minimum Detection Limit: 1 pg/mL

Application: ELISA

Purpose: Human TNF RI (TNFRSF1A) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensitivity: 1 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples20 - 200 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for TNFRSF1A ELISA Kit (ABIN4884842)

Fernández-Juárez, Villacorta Perez, Luño Fernández, Martinez-Martinez, Cachofeiro, Barrio Lucia, Tato Ribera, Mendez Abreu, Cordon, Oliva Dominguez, Praga Terente: "High levels of circulating TNFR1 increase the risk of all-cause mortality and progression of renal disease in type 2 diabetic nephropathy." in: Nephrology (Carlton, Vic.), Vol. 22, Issue 5, pp. 354-360, (2017) (PubMed).

Gao, Camous, Lu, Lim, Larbi, Ng: "Novel inflammatory markers associated with cognitive performance: Singapore Longitudinal Ageing Studies." in: Neurobiology of aging, Vol. 39, pp. 140-6, (2016) (PubMed).

Wu, Ding, Han, Arriens, Wei, Han, Pedroza, Jiang, Anolik, Petri, Sanz, Saxena, Mohan: "Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study." in: Journal of proteome research, Vol. 15, Issue 7, pp. 2102-14, (2016) (PubMed).

Sennikov, Alshevskaya, Shkaruba, Chumasova, Sizikov, Lopatnikova: "Expression of TNF? membrane-bound receptors in the peripheral blood mononuclear cells (PMBC) in rheumatoid arthritis patients." in: Cytokine, Vol. 73, Issue 2, pp. 288-94, (2015) (PubMed).

Bishayi, Bandyopadhyay, Majhi, Adhikary et al.: "Expression of CXCR1 (interleukin-8 receptor) in murine macrophages after staphylococcus aureus infection and its possible implication on intracellular survival correlating with cytokines and ..." in: Inflammation, Vol. 38, Issue 2, pp. 812-27, (2015) (PubMed).

Golikova, Lopatnikova, Kovalevskaya-Kucheryavenko, Nepomnyashih, Sennikov: "Levels of TNF, TNF autoantibodies and soluble TNF receptors in patients with bronchial asthma." in: The Journal of asthma : official journal of the Association for the Care of Asthma, Vol. 50, Issue 7, pp. 705-11, (2014) (PubMed).

Cuchacovich, Hagan, Khan, Richert, Espinoza: "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." in: Clinical rheumatology, Vol. 30, Issue 1, pp. 133-7, (2011) (PubMed).

Issersohn: "[Radiologic examination of the colon with double contrast medium according to the Welin method]." in: Dapim refuiim. Folia medica, Vol. 24, Issue 1, pp. 27-31 (PubMed).

Target Details for TNFRSF1A

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Alternative Name: TNF RI (TNFRSF1A Products)

Synonyms: CD120a ELISA Kit, FPF ELISA Kit, MS5 ELISA Kit, TBP1 ELISA Kit, TNF-R ELISA Kit, TNF-R-I ELISA Kit, TNF-R55 ELISA Kit, TNFAR ELISA Kit, TNFR1 ELISA Kit, TNFR1-d2 ELISA Kit, TNFR55 ELISA Kit, TNFR60 ELISA Kit, p55 ELISA Kit, p55-R ELISA Kit, p60 ELISA Kit, TNF-R1 ELISA Kit, TNF-alphaR1 ELISA Kit, TNFRI ELISA Kit, TNFRp55 ELISA Kit, TNFalpha-R1 ELISA Kit, Tnfr-2 ELISA Kit, Tnfr1 ELISA Kit, fpf ELISA Kit, tbp1 ELISA Kit, p55-r ELISA Kit, tnf-r ELISA Kit, tnfar ELISA Kit, tnfr1 ELISA Kit, cd120a ELISA Kit, tnfr55 ELISA Kit, tnfr60 ELISA Kit, xtnfr1 ELISA Kit, wu:fj65b10 ELISA Kit, zgc:85914 ELISA Kit, TNFR I ELISA Kit, TNFRSF1A ELISA Kit, TNFA ELISA Kit, cTNF ELISA Kit, TNF receptor superfamily member 1A ELISA Kit, tumor necrosis factor receptor superfamily, member 1a ELISA Kit, TNF receptor superfamily member 1A S homeolog ELISA Kit, tumor necrosis factor ELISA Kit, TNFRSF1A ELISA Kit, Tnfrsf1a ELISA Kit, tnfrsf1a.S ELISA Kit, tnfrsf1a ELISA Kit, TNF ELISA Kit

Background: Gene Names: TNFRSF1A TNFAR TNFR1
Protein names: Tumor necrosis factor receptor superfamily member 1A (Tumor necrosis factor receptor 1) (TNF-R1) (Tumor necrosis factor receptor type I) (TNF-RI) (TNFR-I) (p55) (p60) (CD antigen CD120a) [Cleaved into: Tumor necrosis factor receptor superfamily member 1A, membrane form, Tumor necrosis factor-binding protein 1 (TBPI)]

Gene ID: 7132

UniProt: P19438

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway