TRKA ELISA Kit

Catalog No. ABIN4884901

Product Details TRKA ELISA Kit

Target: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2-500 ng/mL

Minimum Detection Limit: 2 ng/mL

Application: ELISA

Purpose: Human TrkA ELISA Kit for serum, plasma, and cell culture supernatant samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human TrkA. Other species not determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for TRKA

Target: TRKA (NTRK1) (Neurotrophic Tyrosine Kinase, Receptor, Type 1 (NTRK1))

Alternative Name: TrkA (NTRK1 Products)

Synonyms: MTC ELISA Kit, TRK ELISA Kit, TRK1 ELISA Kit, TRKA ELISA Kit, Trk-A ELISA Kit, p140-TrkA ELISA Kit, CDHF12 ELISA Kit, CDHR16 ELISA Kit, HSCR1 ELISA Kit, MEN2A ELISA Kit, MEN2B ELISA Kit, MTC1 ELISA Kit, PTC ELISA Kit, RET-ELE1 ELISA Kit, RET51 ELISA Kit, Trk ELISA Kit, C80751 ELISA Kit, Tkr ELISA Kit, TrkA ELISA Kit, trk ELISA Kit, trka ELISA Kit, CTRKA ELISA Kit, trkA ELISA Kit, neurotrophic receptor tyrosine kinase 1 ELISA Kit, ret proto-oncogene ELISA Kit, neurotrophic tyrosine kinase, receptor, type 1 ELISA Kit, NTRK1 ELISA Kit, RET ELISA Kit, Ntrk1 ELISA Kit, ntrk1 ELISA Kit

Gene ID: 4914

UniProt: P04629

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process