CEACAM1 ELISA Kit

Catalog No. ABIN4885168

Product Details CEACAM1 ELISA Kit

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human CEACAM-1 SpeedELISA Kit.

Brand: SpeedELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Characteristics:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details for CEACAM1

Target: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Alternative Name: CEACAM-1 (CEACAM1 Products)

Synonyms: BGP ELISA Kit, BGP1 ELISA Kit, BGPI ELISA Kit, CD66e ELISA Kit, CEA ELISA Kit, zeh0068 ELISA Kit, hm:zeh0068 ELISA Kit, CEACAM8 ELISA Kit, CEACAM1 ELISA Kit, MGC142545 ELISA Kit, BGPR ELISA Kit, Bgp ELISA Kit, CD66a ELISA Kit, Ccam1 ELISA Kit, Bgp1 ELISA Kit, C-CAM ELISA Kit, Cc1 ELISA Kit, Cea-1 ELISA Kit, Cea-7 ELISA Kit, Cea1 ELISA Kit, Cea7 ELISA Kit, Hv-2 ELISA Kit, Hv2 ELISA Kit, MHVR ELISA Kit, MHVR1 ELISA Kit, Mhv-1 ELISA Kit, bb-1 ELISA Kit, mCEA1 ELISA Kit, mmCGM1 ELISA Kit, mmCGM1a ELISA Kit, mmCGM2 ELISA Kit, carcinoembryonic antigen related cell adhesion molecule 1 ELISA Kit, carcinoembryonic antigen related cell adhesion molecule 5 ELISA Kit, carcinoembryonic antigen-related cell adhesion molecule 1 ELISA Kit, carcinoembryonic antigen-related cell adhesion molecule 6 ELISA Kit, CEACAM1 ELISA Kit, CEACAM5 ELISA Kit, ceacam1 ELISA Kit, Ceacam1 ELISA Kit, LOC101103343 ELISA Kit

Gene ID: 634

UniProt: P13688