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ACVRL1 ELISA Kit

ACVRL1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.41-100 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4885419
  • Target See all ACVRL1 ELISA Kits
    ACVRL1 (Activin A Receptor Type II-Like 1 (ACVRL1))
    Reactivity
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.41-100 ng/mL
    Minimum Detection Limit
    0.41 ng/mL
    Application
    ELISA
    Purpose
    Mouse ALK-1 ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product ACVRL1 ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all ACVRL1 ELISA Kits
    ACVRL1 (Activin A Receptor Type II-Like 1 (ACVRL1))
    Alternative Name
    ALK-1 (ACVRL1 Products)
    Synonyms
    ACVRL1 ELISA Kit, vgb ELISA Kit, alk1 ELISA Kit, zehn1109 ELISA Kit, hm:zehn1109 ELISA Kit, ACVRLK1 ELISA Kit, ALK-1 ELISA Kit, ALK1 ELISA Kit, HHT ELISA Kit, HHT2 ELISA Kit, ORW2 ELISA Kit, SKR3 ELISA Kit, TSR-I ELISA Kit, R-3 ELISA Kit, R3 ELISA Kit, SETHKIR ELISA Kit, AI115505 ELISA Kit, AI427544 ELISA Kit, Acvrlk1 ELISA Kit, Alk1 ELISA Kit, activin A receptor like type 1 ELISA Kit, activin A receptor type II-like 1 ELISA Kit, activin A receptor, type II-like 1 ELISA Kit, ACVRL1 ELISA Kit, acvrl1 ELISA Kit, Acvrl1 ELISA Kit
    Gene ID
    20568
    UniProt
    P97430
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