COL18A1 ELISA Kit

Catalog No. ABIN4885503

Product Details COL18A1 ELISA Kit

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2.458-600 ng/mL

Minimum Detection Limit: 2.458 ng/mL

Application: ELISA

Purpose: Mouse Endostatin ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Mouse Endostatin (COL18A1)

Sensitivity: 2.5 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for COL18A1 ELISA Kit (ABIN4885503)

Pan, Luo, Zhou, Han, Zeng: "Potent antitumor activity of the combination of HSV-TK and endostatin by adeno-associated virus vector for bladder cancer in vivo." in: Clinical laboratory, Vol. 59, Issue 9-10, pp. 1147-58, (2013) (PubMed).

Pan, Zhou, Zeng, Han: "Suppression of bladder cancer growth in mice by adeno-associated virus vector-mediated endostatin expression." in: Tumour biology, Vol. 32, Issue 2, pp. 301-10, (2011) (PubMed).

Target Details for COL18A1

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Alternative Name: Endostatin (COL18A1 Products)

Target Type: Chemical