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E-cadherin ELISA Kit
CDH1
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Product Details E-cadherin ELISA Kit
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Target
See all E-cadherin (CDH1) ELISA Kits
E-cadherin (CDH1)
(Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
Reactivity
All reactivities for E-cadherin elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Mouse E-Cadherin SpeedELISA Kit.
Brand
SpeedELISA
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Characteristics
Compressed workflow with only one wash step and three incubation steps 3 hours total processing time Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Streptavidin-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody HRP Conjugated Detection Antibody TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Assay Time
3 h
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 70 μL of the Antibody Cocktail to each well. Incubate 10 minutes at RT. Add 50 μL of sample or standard to each well. Incubate 2 h at RT. Wash Wells Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Expiry Date
6 months
Target Details for E-cadherin
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Target
See all E-cadherin (CDH1) ELISA Kits
E-cadherin (CDH1)
(Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
Alternative Name
E-Cadherin (CDH1 Products )
Synonyms
Arc-1 ELISA Kit, CD324 ELISA Kit, CDHE ELISA Kit, ECAD ELISA Kit, LCAM ELISA Kit, UVO ELISA Kit, E-cadherin ELISA Kit, cdh1 ELISA Kit, si:dz180o5.2 ELISA Kit, uvo ELISA Kit, lcam ELISA Kit, E-Cad ELISA Kit, XBcad ELISA Kit, l-cam ELISA Kit, xcdh1 ELISA Kit, cdhc-A ELISA Kit, xb-cad ELISA Kit, XTCAD-1 ELISA Kit, c-cadherin ELISA Kit, cadherin-1 ELISA Kit, uvomorulin ELISA Kit, XB-cadherin ELISA Kit, arc-1 ELISA Kit, cd324 ELISA Kit, cdhe ELISA Kit, ecad ELISA Kit, AA960649 ELISA Kit, E-cad ELISA Kit, Ecad ELISA Kit, L-CAM ELISA Kit, Um ELISA Kit, cadherin 1 ELISA Kit, si:busm1-180o5.2 ELISA Kit, cadherin 1, type 1, E-cadherin (epithelial) ELISA Kit, cadherin 1, type 1 S homeolog ELISA Kit, CDH1 ELISA Kit, si:busm1-180o5.2 ELISA Kit, cdh1 ELISA Kit, Cdh1 ELISA Kit, cdh1.S ELISA Kit
Gene ID
12550
UniProt
P09803
Pathways
WNT Signaling , Sensory Perception of Sound , Cell-Cell Junction Organization , Tube Formation
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