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FCGR2B ELISA Kit
FCGR2B
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Plasma, Serum
Product Details FCGR2B ELISA Kit
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Target
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FCGR2B
(Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))
Reactivity
All reactivities for FCGR2B elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Mouse Fc gamma RIIB (CD32b) SpeedELISA Kit.
Brand
SpeedELISA
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Characteristics
Compressed workflow with only one wash step and three incubation steps 3 hours total processing time Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Streptavidin-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Capture Antibody HRP Conjugated Detection Antibody TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Assay Time
3 h
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 70 μL of the Antibody Cocktail to each well. Incubate 10 minutes at RT. Add 50 μL of sample or standard to each well. Incubate 2 h at RT. Wash Wells Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Expiry Date
6 months
Target Details for FCGR2B
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Target
See all FCGR2B ELISA Kits
FCGR2B
(Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))
Alternative Name
Fc gamma RIIB (FCGR2B Products )
Synonyms
CD32 ELISA Kit, CD32B ELISA Kit, FCG2 ELISA Kit, FCGR2 ELISA Kit, IGFR2 ELISA Kit, FCGR2B ELISA Kit, AI528646 ELISA Kit, F630109E10Rik ELISA Kit, Fc[g]RII ELISA Kit, FcgRII ELISA Kit, Fcgr2 ELISA Kit, Fcgr2a ELISA Kit, Fcr-2 ELISA Kit, Fcr-3 ELISA Kit, Ly-17 ELISA Kit, Ly-m20 ELISA Kit, LyM-1 ELISA Kit, Lym-1 ELISA Kit, FCRII ELISA Kit, FcRII ELISA Kit, Fc fragment of IgG receptor IIb ELISA Kit, Fc fragment of IgG, low affinity IIb, receptor (CD32) ELISA Kit, Fc receptor, IgG, low affinity IIb ELISA Kit, low affinity immunoglobulin gamma Fc region receptor II ELISA Kit, FCGR2B ELISA Kit, Fcgr2b ELISA Kit, LOC100856270 ELISA Kit
Gene ID
14130
UniProt
P08101
Pathways
Cellular Response to Molecule of Bacterial Origin , Regulation of Leukocyte Mediated Immunity , Production of Molecular Mediator of Immune Response , BCR Signaling
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