beta-Thromboglobulin ELISA Kit

Catalog No. ABIN4885809

Product Details beta-Thromboglobulin ELISA Kit

Target: beta-Thromboglobulin (beta-TG)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Mouse TCK-1 (CXCL7) SpeedELISA Kit.

Brand: SpeedELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Characteristics:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details for beta-Thromboglobulin

Target: beta-Thromboglobulin (beta-TG)

Alternative Name: TCK-1 (beta-TG Products)

Synonyms: B-TG1 ELISA Kit, Beta-TG ELISA Kit, CTAP-III ELISA Kit, CTAP3 ELISA Kit, CTAPIII ELISA Kit, CXCL7 ELISA Kit, LA-PF4 ELISA Kit, LDGF ELISA Kit, MDGF ELISA Kit, NAP-2 ELISA Kit, PBP ELISA Kit, SCYB7 ELISA Kit, TC1 ELISA Kit, TC2 ELISA Kit, TGB ELISA Kit, TGB1 ELISA Kit, THBGB ELISA Kit, THBGB1 ELISA Kit, CTAPIII/NAP-2 ELISA Kit, 2400003M24Rik ELISA Kit, AI854500 ELISA Kit, Cxcl7 ELISA Kit, NAP-2-L1 ELISA Kit, Scyb7 ELISA Kit, b-TG1 ELISA Kit, beta-TG ELISA Kit, Nap-2 ELISA Kit, pro-platelet basic protein ELISA Kit, PPBP ELISA Kit, Ppbp ELISA Kit

Gene ID: 57349

UniProt: Q9EQI5