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TNFRSF21 ELISA Kit

TNFRSF21 Reactivity: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4885864
  • Target See all TNFRSF21 ELISA Kits
    TNFRSF21 (Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21))
    Reactivity
    • 3
    • 2
    • 2
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Rat Death Receptor 6 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Rat Death Receptor 6.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product TNFRSF21 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all TNFRSF21 ELISA Kits
    TNFRSF21 (Tumor Necrosis Factor Receptor Superfamily, Member 21 (TNFRSF21))
    Alternative Name
    DR6 (TNFRSF21 Products)
    Synonyms
    TNFRSF21 ELISA Kit, tnfrsf21 ELISA Kit, MGC146356 ELISA Kit, BM-018 ELISA Kit, CD358 ELISA Kit, DR6 ELISA Kit, AA959878 ELISA Kit, R74815 ELISA Kit, TR7 ELISA Kit, dr6 ELISA Kit, im:6795346 ELISA Kit, wu:fa55e01 ELISA Kit, wu:fb02e11 ELISA Kit, wu:fc29a09 ELISA Kit, wu:fi27h08 ELISA Kit, TNF receptor superfamily member 21 ELISA Kit, tumor necrosis factor receptor superfamily member 21 ELISA Kit, tumor necrosis factor receptor superfamily, member 21 ELISA Kit, TNFRSF21 ELISA Kit, tnfrsf21 ELISA Kit, Tnfrsf21 ELISA Kit
    Gene ID
    316256
    UniProt
    D3ZF92
    Pathways
    Regulation of Lipid Metabolism by PPARalpha
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