NLRP2 ELISA Kit

Catalog No. ABIN4886224

Product Details NLRP2 ELISA Kit

Target: NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))

Binding Specificity: pSer343, total

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Phospho-NBS1 (S343) and Total NBS1 ELISA Kit. This assay semi-quantitatively measures phophorylated NBS1 (Ser343) and Total NBS1 in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human NBS1 phosphorylated at site Serine-343 and total NBS1.

Characteristics:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for NLRP2

Target: NLRP2 (NLR Family, Pyrin Domain Containing 2 (NLRP2))

Alternative Name: NBS1 (NLRP2 Products)

Synonyms: CLR19.9 ELISA Kit, NALP2 ELISA Kit, NBS1 ELISA Kit, PAN1 ELISA Kit, PYPAF2 ELISA Kit, NLRP2 ELISA Kit, E330007A02Rik ELISA Kit, Nalp2 ELISA Kit, NLR family pyrin domain containing 2 ELISA Kit, NACHT, LRR and PYD domains-containing protein 2 ELISA Kit, NLR family pyrin domain containing 7 ELISA Kit, NLR family, pyrin domain containing 2 ELISA Kit, NLRP2 ELISA Kit, LOC100301026 ELISA Kit, LOC100387349 ELISA Kit, NLRP7 ELISA Kit, Nlrp2 ELISA Kit

Gene ID: 55655

UniProt: Q9NX02

Pathways: Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome