VEGFR2/CD309 ELISA Kit

Catalog No. ABIN4886309

Product Details VEGFR2/CD309 ELISA Kit

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Binding Specificity: pTyr

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Phosphotyrosine STAT4 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine STAT4 in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: This ELISA kit recognizes human VEGFR2 phosphotyrosine.

Characteristics:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Alternative Name: VEGFR2 (VEGFR2 Products)

Synonyms: CD309 ELISA Kit, FLK1 ELISA Kit, VEGFR ELISA Kit, VEGFR2 ELISA Kit, 6130401C07 ELISA Kit, Flk-1 ELISA Kit, Flk1 ELISA Kit, Krd-1 ELISA Kit, Ly73 ELISA Kit, VEGFR-2 ELISA Kit, sVEGFR-2 ELISA Kit, Vegfr-2 ELISA Kit, FLK-1 ELISA Kit, flk-1 ELISA Kit, vegfr-2 ELISA Kit, KDR ELISA Kit, FLK/KDR ELISA Kit, flk1 ELISA Kit, flk1b ELISA Kit, kdrb ELISA Kit, si:busm1-205d10.1 ELISA Kit, si:ch211-254j6.1 ELISA Kit, si:ch211-278f21.4 ELISA Kit, wu:fc31a09 ELISA Kit, fk52c05 ELISA Kit, flk ELISA Kit, kdr ELISA Kit, kdra ELISA Kit, vegfr2 ELISA Kit, vegfr4 ELISA Kit, vegr2 ELISA Kit, wu:fk52c05 ELISA Kit, kinase insert domain receptor ELISA Kit, kinase insert domain protein receptor ELISA Kit, vascular endothelial growth factor receptor kdr-like ELISA Kit, kinase insert domain receptor (a type III receptor tyrosine kinase) ELISA Kit, kinase insert domain receptor like ELISA Kit, KDR ELISA Kit, Kdr ELISA Kit, LOC100136647 ELISA Kit, kdr ELISA Kit, kdrl ELISA Kit

Gene ID: 6775

UniProt: Q14765

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling