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CDK4 ELISA Kit

CDK4 Reactivity: Mouse Colorimetric Sandwich ELISA 62.5-4000 pg/mL Plasma, Serum
Catalog No. ABIN4993370
  • Target See all CDK4 ELISA Kits
    CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
    Reactivity
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5-4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Purpose
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CDK4 in Mouse serum, plasma and other biological fluids
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This kit recognizes natural and recombinantMouseCDK4. No significant cross-reactivity or interference between MouseCDK4 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between MouseCDK4 and all the analogues.
    Sensitivity
    37.5 pg/mL
    Components
    Micro ELISA Plate
    Reference Standard
    Concentrated Biotinylated Detection Ab
    Concentrated HRP Conjugate
    Reference Standard & Sample Diluent
    Biotinylated Detection Ab Diluent
    HRP Conjugate Diluent
    Concentrated Wash Buffer (25×)
    Substrate Reagent
    Stop Solutio
    Plate Sealer
    Product Description
    Certificate of Analysis
    Material not included
    Microplate reader with 450nm wavelength filter
    High-precision transferpettor, EP tubes and disposable pipette tips
    37℃ Incubator
    Deionized or distilled water
    Absorbent paper
    Loading slot for Wash Buffer
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  • Comment

    Information on standard material:
    The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

    Information on reagents:
    Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

    Information on antibodies:
    The provided antibodies and their host vary in different kits. All antibodies are affinity purified

    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

    Sample Volume
    100 µL
    Plate
    Pre-coated
    Protocol
    This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CDK4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CDK4and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CDK4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CDK4.You can calculate the concentration of CDK4in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
    Restrictions
    For Research Use only
  • Handling Advice
    All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
    Storage
    4 °C/-20 °C
    Storage Comment
    The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
  • Target See all CDK4 ELISA Kits
    CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
    Alternative Name
    Cyclin Dependent Kinase 4 (CDK4) (CDK4 Products)
    Synonyms
    zgc:153726 ELISA Kit, CMM3 ELISA Kit, PSK-J3 ELISA Kit, Crk3 ELISA Kit, 8-6 ELISA Kit, CDK4 ELISA Kit, CDK4/6 ELISA Kit, CG5072 ELISA Kit, Cdk4/6 ELISA Kit, DmCdk4 ELISA Kit, Dmel\\CG5072 ELISA Kit, Pk53C ELISA Kit, Pk?7 ELISA Kit, cdk ELISA Kit, cdk4 ELISA Kit, cdk4/6 ELISA Kit, dCdk4 ELISA Kit, l(2)05428 ELISA Kit, l(2)0671 ELISA Kit, l(2)k06503 ELISA Kit, l(2)s4639 ELISA Kit, l(2)sh0671 ELISA Kit, xcdk4 ELISA Kit, cyclin-dependent kinase 4 ELISA Kit, cyclin dependent kinase 4 ELISA Kit, Cyclin-dependent kinase 4 ELISA Kit, cyclin-dependent kinase 4 L homeolog ELISA Kit, cdk4 ELISA Kit, CDK4 ELISA Kit, Cdk4 ELISA Kit, cdk4.L ELISA Kit
    Pathways
    Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
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