IL-18 ELISA Kit
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- Target See all IL-18 (IL18) ELISA Kits
- IL-18 (IL18) (Interleukin 18 (IL18))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 15.6-1000 pg/mL
- Minimum Detection Limit
- 15.6 pg/mL
- Application
- ELISA
- Purpose
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-18 concentrations in serum, plasma and other biological fluids.
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Sensitivity
- < 9.4 pg/mL
- Components
- plate, standard, diluent, antibodies
- Material not included
- pipettes, tubes, reader, incubator
- Featured
- Discover our best selling IL18 ELISA Kit
- Top Product
- Discover our top product IL18 ELISA Kit
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Interleukin 18 (IL18) ELISA Kit
IL18 Reactivity: Human Colorimetric Sandwich ELISA 31.25-2000 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Interleukin 18 (IL18) ELISA KitIL18 Reactivity: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate, Urine
Interleukin 18 (IL18) ELISA KitIL18 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 Reactivity: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitHigh Sensitivity IL18 Reactivity: Human Colorimetric Sandwich ELISA 1.563 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 Reactivity: Rabbit Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 Reactivity: Chicken Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 18 (IL18) ELISA KitIL18 Reactivity: Guinea Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- Sample Volume
- 100 μL
- Assay Time
- 3 - 4 h
- Plate
- Pre-coated
- Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-18 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-18 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-18 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-18 can be calculated using the standard curve.
- Restrictions
- For Research Use only
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- Precaution of Use
- protect your eyes
- Storage
- 4 °C
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- Target See all IL-18 (IL18) ELISA Kits
- IL-18 (IL18) (Interleukin 18 (IL18))
- Alternative Name
- IL-18 (Interleukin 18) (IL18 Products)
- Synonyms
- Igif ELISA Kit, Il-18 ELISA Kit, IL-18 ELISA Kit, IGIF ELISA Kit, ChIL-18 ELISA Kit, IL-1g ELISA Kit, IL1F4 ELISA Kit, IL18 ELISA Kit, interleukin 18 ELISA Kit, Il18 ELISA Kit, IL18 ELISA Kit
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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